{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ha NS"],"funding":["U.S. Department of Energy","National Institutes of Health","NIGMS NIH HHS"],"pagination":["3361-3369"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10484474"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(15)"],"pubmed_abstract":["Mass spectrometry (MS) enables detection of different chemical species with a very high specificity; however, it can be limited by its throughput. Integrating MS with microfluidics has a tremendous potential to improve throughput and accelerate biochemical research. In this work, we introduce Drop-NIMS, a combination of a passive droplet loading microfluidic device and a matrix-free MS laser desorption ionization technique called nanostructure-initiator mass spectrometry (NIMS). This platform combines different droplets at random to generate a combinatorial library of enzymatic reactions that are deposited directly on the NIMS surface without requiring additional sample handling. The enzyme reaction products are then detected with MS. Drop-NIMS was used to rapidly screen enzymatic reaction"],"journal":["Lab on a chip"],"pubmed_title":["A combinatorial droplet microfluidic device integrated with mass spectrometry for enzyme screening."],"pmcid":["PMC10484474"],"funding_grant_id":["DE-AC02-05CH11231","T32 GM008349","NIH 5 T32 GM008349","DE-FC0207ER64494"],"pubmed_authors":["Adams PD","Kuch N","Bowen BP","Deng K","Song F","Kim PW","Kutschke M","Parker A","Ha NS","Northen TR","de Raad M","Andeer P","Onley JR","Gupta K","Fox B"],"additional_accession":[]},"is_claimable":false,"name":"A combinatorial droplet microfluidic device integrated with mass spectrometry for enzyme screening.","description":"Mass spectrometry (MS) enables detection of different chemical species with a very high specificity; however, it can be limited by its throughput. Integrating MS with microfluidics has a tremendous potential to improve throughput and accelerate biochemical research. In this work, we introduce Drop-NIMS, a combination of a passive droplet loading microfluidic device and a matrix-free MS laser desorption ionization technique called nanostructure-initiator mass spectrometry (NIMS). This platform combines different droplets at random to generate a combinatorial library of enzymatic reactions that are deposited directly on the NIMS surface without requiring additional sample handling. The enzyme reaction products are then detected with MS. Drop-NIMS was used to rapidly screen enzymatic reaction","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2025-06-01T01:16:41.907Z","creation":"2025-06-01T01:16:41.907Z"},"accession":"S-EPMC10484474","cross_references":{"pubmed":["37401915"],"doi":["10.1039/d2lc00980c"]}}