biostudies-literatureUnknown13Tian CThe emergence of carbapenemase-producing <i>Acinetobacter</i> spp. has been widely reported and become a global threat. However, carbapenem-resistant <i>A. johnsonii</i> strains are relatively rare and without comprehensive genetic structure analysis, especially for isolates collected from human specimen. Here, one <i>A. johnsonii</i> AYTCM strain, co-producing NDM-1, OXA-58, and PER-1 enzymes, was isolated from sputum in China in 2018. Antimicrobial susceptibility testing showed that it was resistant to meropenem, imipenem, ceftazidime, ciprofloxacin, and cefoperazone/sulbactam. Whole-genome sequencing and bioinformatic analysis revealed that it possessed 11 plasmids. <i>bla</i> _OXA-58 and <i>bla</i> _PER-1 genes were located in the pAYTCM-1 plasmid. Especially, a comFrontiers in cellular and infection microbiology1227063https://www.ebi.ac.uk/biostudies/studies/S-EPMC10486904biostudies-literatureComplete genetic characterization of carbapenem-resistant <i>Acinetobacter johnsonii</i>, co-producing NDM-1, OXA-58, and PER-1 in a patient source.PMC10486904Huang DFan XRen LWang STian CYing JFu LBai YMa TSong JZhao YfalseComplete genetic characterization of carbapenem-resistant <i>Acinetobacter johnsonii</i>, co-producing NDM-1, OXA-58, and PER-1 in a patient source.The emergence of carbapenemase-producing <i>Acinetobacter</i> spp. has been widely reported and become a global threat. However, carbapenem-resistant <i>A. johnsonii</i> strains are relatively rare and without comprehensive genetic structure analysis, especially for isolates collected from human specimen. Here, one <i>A. johnsonii</i> AYTCM strain, co-producing NDM-1, OXA-58, and PER-1 enzymes, was isolated from sputum in China in 2018. Antimicrobial susceptibility testing showed that it was resistant to meropenem, imipenem, ceftazidime, ciprofloxacin, and cefoperazone/sulbactam. Whole-genome sequencing and bioinformatic analysis revealed that it possessed 11 plasmids. <i>bla</i> _OXA-58 and <i>bla</i> _PER-1 genes were located in the pAYTCM-1 plasmid. Especially, a com2023-01-01T00:00:00Z20232026-04-29T02:18:38.811Z2025-02-19T04:01:34.231ZS-EPMC104869043769216210.3389/fcimb.2023.1227063