biostudies-literaturePeng CCEuropean Research CouncilDeutsche Forschungsgemeinschaft27520-27524https://www.ebi.ac.uk/biostudies/studies/S-EPMC10501047biostudies-literatureUnknown13(39)The choanoflagellate <i>Salpingoeca rosetta</i> transitions from unicellular to multicellular forms in the presence of bacterial signaling molecules, such as sulfonosphingolipids (RIFs). We set out to characterize the abundance of RIF-like molecules within five different Bacteroidetes strains belonging to different genera. While four strains exhibited similar sulfonosphingolipid profiles with sulfobacin A as the dominant feature, the composition in <i>Z. uliginosa</i> differed distinctively. Targeted isolation yielded four sulfonosphingolipids, including the previously reported flavocristamide A. While none of the sulfonosphingolipids induced rosette formation, a negative impact on choanoflagellate growth and cell density was observed. In contrast, supernatant extracts of <i>Zobellia</i> dRSC advancesIsolation of sulfonosphingolipids from the rosette-inducing bacterium <i>Zobellia uliginosa</i> and evaluation of their rosette-inducing activity.PMC10501047BE 4799/2-1802736Dormanns NPeng CCBeemelmanns CRegestein LfalseIsolation of sulfonosphingolipids from the rosette-inducing bacterium <i>Zobellia uliginosa</i> and evaluation of their rosette-inducing activity.The choanoflagellate <i>Salpingoeca rosetta</i> transitions from unicellular to multicellular forms in the presence of bacterial signaling molecules, such as sulfonosphingolipids (RIFs). We set out to characterize the abundance of RIF-like molecules within five different Bacteroidetes strains belonging to different genera. While four strains exhibited similar sulfonosphingolipid profiles with sulfobacin A as the dominant feature, the composition in <i>Z. uliginosa</i> differed distinctively. Targeted isolation yielded four sulfonosphingolipids, including the previously reported flavocristamide A. While none of the sulfonosphingolipids induced rosette formation, a negative impact on choanoflagellate growth and cell density was observed. In contrast, supernatant extracts of <i>Zobellia</i> d2023-01-01T00:00:00Z2023 Sep2025-04-06T18:32:25.577Z2025-04-06T18:32:25.577ZS-EPMC105010473772082710.1039/d3ra04314b