biostudies-literatureWarecki BNIGMS NIH HHSeadh0601https://www.ebi.ac.uk/biostudies/studies/S-EPMC10501416biostudies-literatureUnknown16(792)During cytokinesis, the cell membrane furrows inward along a cleavage plane. The positioning of the cleavage plane is critical to faithful cell division and is determined by the Rho guanine nucleotide exchange factor (RhoGEF)-mediated activation of the small guanosine triphosphatase RhoA and the conserved motor protein complex centralspindlin. Here, we explored whether and how centralspindlin mediates the positioning of RhoGEF. In dividing neuroblasts from <i>Drosophila melanogaster</i>, we observed that immediately before cleavage, first centralspindlin and then RhoGEF localized to the sites where cleavage subsequently initiated. Using in vitro assays with purified <i>Drosophila</i> proteins and stabilized microtubules, we found that centralspindlin directly transported RhoGEF as cargo alScience signalingCentralspindlin-mediated transport of RhoGEF positions the cleavage plane for cytokinesis.PMC10501416P20 GM103466R35 GM139595Tao LWarecki BfalseCentralspindlin-mediated transport of RhoGEF positions the cleavage plane for cytokinesis.During cytokinesis, the cell membrane furrows inward along a cleavage plane. The positioning of the cleavage plane is critical to faithful cell division and is determined by the Rho guanine nucleotide exchange factor (RhoGEF)-mediated activation of the small guanosine triphosphatase RhoA and the conserved motor protein complex centralspindlin. Here, we explored whether and how centralspindlin mediates the positioning of RhoGEF. In dividing neuroblasts from <i>Drosophila melanogaster</i>, we observed that immediately before cleavage, first centralspindlin and then RhoGEF localized to the sites where cleavage subsequently initiated. Using in vitro assays with purified <i>Drosophila</i> proteins and stabilized microtubules, we found that centralspindlin directly transported RhoGEF as cargo al2023-01-01T00:00:00Z2023 Jul2026-06-01T16:17:30.237Z2025-04-06T11:37:27.921ZS-EPMC105014163740222410.1126/scisignal.adh0601