{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Martinez-Balsalobre E"],"funding":["Ministry of Science and Innovation. Spain","Ministry of Economy and Competitiveness | Instituto de Salud Carlos III","Fundación Ramón Areces Consejeria de Sanidad de la Región de Murcia. ZEBER"],"pagination":["5912"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10516865"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Telomerase RNA (TERC) has a noncanonical function in myelopoiesis binding to a consensus DNA binding sequence and attracting RNA polymerase II (RNA Pol II), thus facilitating myeloid gene expression. The CR4/CR5 domain of TERC is known to play this role, since a mutation of this domain found in dyskeratosis congenita (DC) patients decreases its affinity for RNA Pol II, impairing its myelopoietic activity as a result. In this study, we report that two aptamers, short single-stranded oligonucleotides, based on the CR4/CR5 domain were able to increase myelopoiesis without affecting erythropoiesis in zebrafish. Mechanistically, the aptamers functioned as full terc; that is, they increased the expression of master myeloid genes, independently of endogenous terc, by interacting with RNA Pol II a"],"journal":["Nature communications"],"pubmed_title":["Telomerase RNA-based aptamers restore defective myelopoiesis in congenital neutropenic syndromes."],"pmcid":["PMC10516865"],"funding_grant_id":["PI19/00188","PI22/00861"],"pubmed_authors":["Garcia-Moreno D","Garcia-Castillo J","Mulero V","Naranjo-Sanchez E","Cayuela ML","Fernandez-Lajarin M","Alcaraz-Perez F","Martinez-Balsalobre E","Blasco MA"],"additional_accession":[]},"is_claimable":false,"name":"Telomerase RNA-based aptamers restore defective myelopoiesis in congenital neutropenic syndromes.","description":"Telomerase RNA (TERC) has a noncanonical function in myelopoiesis binding to a consensus DNA binding sequence and attracting RNA polymerase II (RNA Pol II), thus facilitating myeloid gene expression. The CR4/CR5 domain of TERC is known to play this role, since a mutation of this domain found in dyskeratosis congenita (DC) patients decreases its affinity for RNA Pol II, impairing its myelopoietic activity as a result. In this study, we report that two aptamers, short single-stranded oligonucleotides, based on the CR4/CR5 domain were able to increase myelopoiesis without affecting erythropoiesis in zebrafish. Mechanistically, the aptamers functioned as full terc; that is, they increased the expression of master myeloid genes, independently of endogenous terc, by interacting with RNA Pol II a","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2026-06-04T06:33:00.344Z","creation":"2024-11-15T17:13:05.88Z"},"accession":"S-EPMC10516865","cross_references":{"pubmed":["37737237"],"doi":["10.1038/s41467-023-41472-7"]}}