{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li D"],"funding":["Intramural NIH HHS","U.S. Department of Health & Human Services | NIH | National Cancer Institute","NCI NIH HHS"],"pagination":["5920"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10517151"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. In solid tumors, the B7-H3 transmembrane protein is an emerging target that harbours two distinct epitope motifs, IgC and IgV, in its ectodomain. Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. These CAR-T cells are characterized by highly activated T cell signaling and significant tumor infiltration. Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the"],"journal":["Nature communications"],"pubmed_title":["Camel nanobody-based B7-H3 CAR-T cells show high efficacy against large solid tumours."],"pmcid":["PMC10517151"],"funding_grant_id":["Z01 BC010891","HHSN261201800001C","ZIA BC010891"],"pubmed_authors":["Liang T","Li D","Ren H","Edmondson E","Wang R","Khan J","Ho M","Tai CH","Ni W","Park C","Zhou J","Mackay S","Croix BS"],"additional_accession":[]},"is_claimable":false,"name":"Camel nanobody-based B7-H3 CAR-T cells show high efficacy against large solid tumours.","description":"Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. In solid tumors, the B7-H3 transmembrane protein is an emerging target that harbours two distinct epitope motifs, IgC and IgV, in its ectodomain. Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. These CAR-T cells are characterized by highly activated T cell signaling and significant tumor infiltration. Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2026-07-14T18:06:32.234Z","creation":"2025-04-06T07:42:08.95Z"},"accession":"S-EPMC10517151","cross_references":{"pubmed":["37739951"],"doi":["10.1038/s41467-023-41631-w"]}}