{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liang N"],"funding":["National Institutes of Health"],"pagination":["2598-2611"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10517232"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["153(9)"],"pubmed_abstract":["<h4>Background</h4>Donor human milk banks use Holder pasteurization (HoP; 62.5°C, 30 min) to reduce pathogens in donor human milk, but this process damages some bioactive milk proteins.<h4>Objectives</h4>We aimed to determine minimal parameters for high-pressure processing (HPP) to achieve >5-log reductions of relevant bacteria in human milk and how these parameters affect an array of bioactive proteins.<h4>Methods</h4>Pooled raw human milk inoculated with relevant pathogens (Enterococcus faecium, Staphylococcus aureus, Listeria monocytogenes, Cronobacter sakazakii) or microbial quality indicators (Bacillus subtilis and Paenibacillus spp. spores) at 7 log CFU/mL was processed at 300-500 MPa at 16-19°C (due to adiabatic heating) for 1-9 min. Surviving microbes were enumerated using standard"],"journal":["The Journal of nutrition"],"pubmed_title":["High-Pressure Processing of Human Milk: A Balance between Microbial Inactivation and Bioactive Protein Preservation."],"pmcid":["PMC10517232"],"funding_grant_id":["5R01HD106140"],"pubmed_authors":["Lowder A","Burroughs S","Mohamed HM","Waite-Cusic J","Dallas DC","Liang N","Kim BJ"],"additional_accession":[]},"is_claimable":false,"name":"High-Pressure Processing of Human Milk: A Balance between Microbial Inactivation and Bioactive Protein Preservation.","description":"<h4>Background</h4>Donor human milk banks use Holder pasteurization (HoP; 62.5°C, 30 min) to reduce pathogens in donor human milk, but this process damages some bioactive milk proteins.<h4>Objectives</h4>We aimed to determine minimal parameters for high-pressure processing (HPP) to achieve >5-log reductions of relevant bacteria in human milk and how these parameters affect an array of bioactive proteins.<h4>Methods</h4>Pooled raw human milk inoculated with relevant pathogens (Enterococcus faecium, Staphylococcus aureus, Listeria monocytogenes, Cronobacter sakazakii) or microbial quality indicators (Bacillus subtilis and Paenibacillus spp. spores) at 7 log CFU/mL was processed at 300-500 MPa at 16-19°C (due to adiabatic heating) for 1-9 min. Surviving microbes were enumerated using standard","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2025-04-22T15:37:55.504Z","creation":"2025-04-06T01:25:01.125Z"},"accession":"S-EPMC10517232","cross_references":{"pubmed":["37423385"],"doi":["10.1016/j.tjnut.2023.07.001"]}}