biostudies-literatureSun XBritish Heart Foundation1867-1886https://www.ebi.ac.uk/biostudies/studies/S-EPMC10521807biostudies-literatureUnknown43(10)<h4>Background</h4>Tertiary lymphoid organs (TLOs) are ectopic lymphoid organs developed in nonlymphoid tissues with chronic inflammation, but little is known about their existence in different types of vascular diseases and the mechanism that mediated their development.<h4>Methods</h4>To take advantage of single-cell RNA sequencing techniques, we integrated 28 single-cell RNA sequencing data sets containing 5 vascular disease models (atherosclerosis, abdominal aortic aneurysm, intimal hyperplasia, isograft, and allograft) to explore TLOs existence and environment supporting its growth systematically. We also searched Medline, Embase, PubMed, and Web of Science from inception to January 2022 for published histological images of vascular remodeling for histological evidence to support TLO gArteriosclerosis, thrombosis, and vascular biologyMeta-Analysis of Single-Cell RNA-Seq Data Reveals the Mechanism of Formation and Heterogeneity of Tertiary Lymphoid Organ in Vascular Disease.PMC10521807PG/15/86/31723PG/20/10458PG/15/11/31279Wu JLiu LLu YWu QXu QWen QCai JSun XYuan HShi YXiao QWeng CLi ZHe THuang WTang YfalseMeta-Analysis of Single-Cell RNA-Seq Data Reveals the Mechanism of Formation and Heterogeneity of Tertiary Lymphoid Organ in Vascular Disease.<h4>Background</h4>Tertiary lymphoid organs (TLOs) are ectopic lymphoid organs developed in nonlymphoid tissues with chronic inflammation, but little is known about their existence in different types of vascular diseases and the mechanism that mediated their development.<h4>Methods</h4>To take advantage of single-cell RNA sequencing techniques, we integrated 28 single-cell RNA sequencing data sets containing 5 vascular disease models (atherosclerosis, abdominal aortic aneurysm, intimal hyperplasia, isograft, and allograft) to explore TLOs existence and environment supporting its growth systematically. We also searched Medline, Embase, PubMed, and Web of Science from inception to January 2022 for published histological images of vascular remodeling for histological evidence to support TLO g2023-01-01T00:00:00Z2023 Oct2026-07-14T18:39:01.586Z2025-04-19T07:13:58.951ZS-EPMC105218073758913410.1161/ATVBAHA.123.318762