biostudies-literatureWang JNational Natural Science Foundation of China3487https://www.ebi.ac.uk/biostudies/studies/S-EPMC10528286biostudies-literatureUnknown12(18)Malto-oligosaccharides (MOSs) from starch conversion is advantageous for food and pharmaceutical applications. In this study, an efficient malto-oligosaccharide-forming α-amylase AmyCf was identified from myxobacter <i>Cystobacter</i> sp. strain CF23. AmyCf is composed of 417 amino acids with N-terminal 41 amino acids as the signal peptide, and conserved glycoside hydrolase family 13 (GH13) catalytic module and predicted C-terminal domain with β-sheet structure are also identified. Phylogenetic and functional analysis demonstrated that AmyCf is a novel member of GH13_6 subfamily. The special activity of AmyCf toward soluble starch and raw wheat starch is 9249 U/mg and 11 U/mg, respectively. AmyCf has broad substrate specificity toward different types of starches without requiring Ca<sup>2+Foods (Basel, Switzerland)Identification and Characterization of Novel Malto-Oligosaccharide-Forming Amylase AmyCf from <i>Cystobacter</i> sp. Strain CF23.PMC1052828632070027322700663217012332270060Wang PLi ZLei JHuang YCui ZZhang LLuo XZhan LWang JYe XZhong LfalseIdentification and Characterization of Novel Malto-Oligosaccharide-Forming Amylase AmyCf from <i>Cystobacter</i> sp. Strain CF23.Malto-oligosaccharides (MOSs) from starch conversion is advantageous for food and pharmaceutical applications. In this study, an efficient malto-oligosaccharide-forming α-amylase AmyCf was identified from myxobacter <i>Cystobacter</i> sp. strain CF23. AmyCf is composed of 417 amino acids with N-terminal 41 amino acids as the signal peptide, and conserved glycoside hydrolase family 13 (GH13) catalytic module and predicted C-terminal domain with β-sheet structure are also identified. Phylogenetic and functional analysis demonstrated that AmyCf is a novel member of GH13_6 subfamily. The special activity of AmyCf toward soluble starch and raw wheat starch is 9249 U/mg and 11 U/mg, respectively. AmyCf has broad substrate specificity toward different types of starches without requiring Ca<sup>2+2023-01-01T00:00:00Z2023 Sep2026-04-08T13:29:32.18Z2025-02-19T04:08:18.164ZS-EPMC105282863776119810.3390/foods12183487