{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["He Y"],"funding":["NHGRI NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["10655-10663"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10528367"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["95(28)"],"pubmed_abstract":["Mass spectrometry (MS)-based proteomics is a powerful technology to globally profile protein abundances, activities, interactions, and modifications. The extreme complexity of proteomics samples, which often contain hundreds of thousands of analytes, necessitates continuous development of MS techniques and instrumentation to improve speed, sensitivity, precision, and accuracy, among other analytical characteristics. Here, we systematically evaluated the Orbitrap Ascend Tribrid mass spectrometer in the context of shotgun proteomics, and we compared its performance to that of the previous generation of Tribrid instruments─the Orbitrap Eclipse. The updated architecture of the Orbitrap Ascend includes a second ion-routing multipole (IRM) in front of the redesigned C-trap/Orbitrap and a new ion"],"journal":["Analytical chemistry"],"pubmed_title":["Evaluation of the Orbitrap Ascend Tribrid Mass Spectrometer for Shotgun Proteomics."],"pmcid":["PMC10528367"],"funding_grant_id":["T32 HG002760","P41 GM108538"],"pubmed_authors":["Zabrouskov V","Westphall MS","Bergen D","He Y","McAlister GC","Huang J","Peters-Clarke TM","Senko MW","Shishkova E","Brademan DR","Huguet R","Coon JJ"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of the Orbitrap Ascend Tribrid Mass Spectrometer for Shotgun Proteomics.","description":"Mass spectrometry (MS)-based proteomics is a powerful technology to globally profile protein abundances, activities, interactions, and modifications. The extreme complexity of proteomics samples, which often contain hundreds of thousands of analytes, necessitates continuous development of MS techniques and instrumentation to improve speed, sensitivity, precision, and accuracy, among other analytical characteristics. Here, we systematically evaluated the Orbitrap Ascend Tribrid mass spectrometer in the context of shotgun proteomics, and we compared its performance to that of the previous generation of Tribrid instruments─the Orbitrap Eclipse. The updated architecture of the Orbitrap Ascend includes a second ion-routing multipole (IRM) in front of the redesigned C-trap/Orbitrap and a new ion","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2025-04-26T08:07:26.471Z","creation":"2025-04-06T12:34:02.076Z"},"accession":"S-EPMC10528367","cross_references":{"pubmed":["37389810"],"doi":["10.1021/acs.analchem.3c01155"]}}