biostudies-literatureUnknown19Guo WIn this study, the effect of Douchi extract (<i>DWE</i>) on α-glucosidase and angiotensin-converting enzymes (ACE) were investigated, and several novel peptides with inhibitory activity against α-glucosidase and ACE were identified using peptidomics approach based on UPLC-MS/MS. The average inhibition rates of <i>DWE</i> on α-glucosidase and ACE were 73.75-78.10% and 4.56-27.07%, respectively. In the <i>DWE</i>, a total of 710 peptides were detected. Two novel peptides with potential inhibitory activity against α-glucosidase were identified using the correlation analysis, database alignment and molecular docking methods. They were DVFRAIPSEVL and DRPSINGLAGAN, with the IC₅₀ values of 0.121 and 0.128 mg/mL, respectively. Also, four novel peptides with potential inhibitory activitFood chemistry: X100779https://www.ebi.ac.uk/biostudies/studies/S-EPMC10534093biostudies-literatureIdentification of novel α-glucosidase and ACE inhibitory peptides from Douchi using peptidomics approach and molecular docking.PMC10534093Lin QJiang LRen KFu XLong ZGuo WXiao YWu YfalseIdentification of novel α-glucosidase and ACE inhibitory peptides from Douchi using peptidomics approach and molecular docking.In this study, the effect of Douchi extract (<i>DWE</i>) on α-glucosidase and angiotensin-converting enzymes (ACE) were investigated, and several novel peptides with inhibitory activity against α-glucosidase and ACE were identified using peptidomics approach based on UPLC-MS/MS. The average inhibition rates of <i>DWE</i> on α-glucosidase and ACE were 73.75-78.10% and 4.56-27.07%, respectively. In the <i>DWE</i>, a total of 710 peptides were detected. Two novel peptides with potential inhibitory activity against α-glucosidase were identified using the correlation analysis, database alignment and molecular docking methods. They were DVFRAIPSEVL and DRPSINGLAGAN, with the IC₅₀ values of 0.121 and 0.128 mg/mL, respectively. Also, four novel peptides with potential inhibitory activit2023-01-01T00:00:00Z2023 Oct2026-04-29T02:30:48.306Z2025-04-07T01:28:47.981ZS-EPMC105340933778023610.1016/j.fochx.2023.100779