biostudies-literatureSayuti NHUniversiti Kebangsaan Malaysia6726https://www.ebi.ac.uk/biostudies/studies/S-EPMC10535396biostudies-literatureUnknown28(18)Neuronal models are an important tool in neuroscientific research. Hydrogen peroxide (H₂O₂), a major risk factor of neuronal oxidative stress, initiates a cascade of neuronal cell death. <i>Polygonum minus</i> Huds, known as 'kesum', is widely used in traditional medicine. <i>P. minus</i> has been reported to exhibit a few medicinal and pharmacological properties. The current study aimed to investigate the neuroprotective effects of <i>P. minus</i> ethanolic extract (PMEE) on H₂O₂-induced neurotoxicity in SH-SY5Y cells. LC-MS/MS revealed the presence of 28 metabolites in PMEE. Our study showed that the PMEE provided neuroprotection against H₂O₂-induced oxidative stress by activating the Nrf2/ARE, NF-κB/IκB and MAPK signalingMolecules (Basel, Switzerland)Ethanolic Extract of <i>Polygonum minus</i> Protects Differentiated Human Neuroblastoma Cells (SH-SY5Y) against H₂O₂-Induced Oxidative Stress.PMC10535396GGPM-2020-037Tan JKMukhtar SSarian MNParveen HSayuti NHBaharum SNAlsharif MASaad NHamezah HSBunawan HZulkefli NAhmed QUfalseEthanolic Extract of <i>Polygonum minus</i> Protects Differentiated Human Neuroblastoma Cells (SH-SY5Y) against H₂O₂-Induced Oxidative Stress.Neuronal models are an important tool in neuroscientific research. Hydrogen peroxide (H₂O₂), a major risk factor of neuronal oxidative stress, initiates a cascade of neuronal cell death. <i>Polygonum minus</i> Huds, known as 'kesum', is widely used in traditional medicine. <i>P. minus</i> has been reported to exhibit a few medicinal and pharmacological properties. The current study aimed to investigate the neuroprotective effects of <i>P. minus</i> ethanolic extract (PMEE) on H₂O₂-induced neurotoxicity in SH-SY5Y cells. LC-MS/MS revealed the presence of 28 metabolites in PMEE. Our study showed that the PMEE provided neuroprotection against H₂O₂-induced oxidative stress by activating the Nrf2/ARE, NF-κB/IκB and MAPK signaling2023-01-01T00:00:00Z2023 Sep2025-04-04T13:32:29.674Z2025-02-19T00:00:41.047ZS-EPMC105353963776450210.3390/molecules28186726