biostudies-literatureDelgado-Garcia MConsejo Nacional de Ciencia y TecnologíaCONACYT2344https://www.ebi.ac.uk/biostudies/studies/S-EPMC10537243biostudies-literatureUnknown11(9)Natural pigments from haloarchaea are of great interest; bacterioruberin is the major pigment, it shows higher antioxidant power when compared with β-carotene. However, characterization of bacterioruberin and its isomers along with its antioxidant and the matrix metallopeptidase 9 (MMP-9) inhibition activities in extracts from <i>Natronoccoccus</i> sp. TC6 and <i>Halorubrum tebenquichense</i> SU10 was not previously described, being the aim of this work. The carotenoids profile was performed by UV-Vis spectrophotometry, thin-layer chromatography, nuclear magnetic resonance spectroscopy, and high-resolution mass spectrometry (UPLC-ESI-MS/MS). Antioxidant capacity was determined for DPPH, ABTS, and FRAP. In addition, MMP-9 inhibition was studied using docking simulations. The carotenoid profMicroorganismsIdentification, Antioxidant Capacity, and Matrix Metallopeptidase 9 (MMP-9) In Silico Inhibition of Haloarchaeal Carotenoids from <i>Natronococcus</i> sp. and <i>Halorubrum tebenquichense</i>.PMC10537243423456CVU 423456Rodriguez JAAguilar CNMateos-Diaz JCDelgado-Garcia MMuller-Santos MGomez-Secundino OCamacho-Ruiz RMfalseIdentification, Antioxidant Capacity, and Matrix Metallopeptidase 9 (MMP-9) In Silico Inhibition of Haloarchaeal Carotenoids from <i>Natronococcus</i> sp. and <i>Halorubrum tebenquichense</i>.Natural pigments from haloarchaea are of great interest; bacterioruberin is the major pigment, it shows higher antioxidant power when compared with β-carotene. However, characterization of bacterioruberin and its isomers along with its antioxidant and the matrix metallopeptidase 9 (MMP-9) inhibition activities in extracts from <i>Natronoccoccus</i> sp. TC6 and <i>Halorubrum tebenquichense</i> SU10 was not previously described, being the aim of this work. The carotenoids profile was performed by UV-Vis spectrophotometry, thin-layer chromatography, nuclear magnetic resonance spectroscopy, and high-resolution mass spectrometry (UPLC-ESI-MS/MS). Antioxidant capacity was determined for DPPH, ABTS, and FRAP. In addition, MMP-9 inhibition was studied using docking simulations. The carotenoid prof2023-01-01T00:00:00Z2023 Sep2026-05-13T14:38:25.804Z2025-02-19T00:01:14.793ZS-EPMC105372433776418810.3390/microorganisms11092344