{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(9)"],"submitter":["Guidolin FR"],"pubmed_abstract":["Accidents with snakes are responsible for about 32,000 deaths annually in sub-Saharan Africa, caused mostly by snakes from the genus <i>Bitis</i>, in particular <i>Bitis arietans</i>. <i>B. arietans</i> venom is composed of a complex mixture of toxins, mainly metalloproteases, serine proteases, phospholipases, lectins, and disintegrins. In this work, we compared two approaches to anti-<i>B</i>. <i>arietans</i> antivenom production: immunization with crude snake venom (\"traditional approach\") and immunization with selected key toxins isolated from the snake venom (\"toxin oriented\" approach). Fractions from <i>B. arietans</i> venom were isolated by size exclusion chromatography. Crude venom and samples containing serine proteases or metalloproteases were selected for the immunization of BALB"],"journal":["Toxins"],"pagination":["584"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10537286"],"repository":["biostudies-literature"],"pubmed_title":["Comparing Traditional and Toxin-Oriented Approaches towards Antivenom Production against <i>Bitis arietans</i> Snake Venom."],"pmcid":["PMC10537286"],"pubmed_authors":["da Silva WD","de Godoi KS","Portaro FCV","Iwai LK","Spencer PJ","Megale AAA","da Silva CCF","Kodama RT","Cajado-Carvalho D","Guidolin FR"],"additional_accession":[]},"is_claimable":false,"name":"Comparing Traditional and Toxin-Oriented Approaches towards Antivenom Production against <i>Bitis arietans</i> Snake Venom.","description":"Accidents with snakes are responsible for about 32,000 deaths annually in sub-Saharan Africa, caused mostly by snakes from the genus <i>Bitis</i>, in particular <i>Bitis arietans</i>. <i>B. arietans</i> venom is composed of a complex mixture of toxins, mainly metalloproteases, serine proteases, phospholipases, lectins, and disintegrins. In this work, we compared two approaches to anti-<i>B</i>. <i>arietans</i> antivenom production: immunization with crude snake venom (\"traditional approach\") and immunization with selected key toxins isolated from the snake venom (\"toxin oriented\" approach). Fractions from <i>B. arietans</i> venom were isolated by size exclusion chromatography. Crude venom and samples containing serine proteases or metalloproteases were selected for the immunization of BALB","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2025-07-06T03:04:22.179Z","creation":"2025-04-07T13:38:40.51Z"},"accession":"S-EPMC10537286","cross_references":{"pubmed":["37756010"],"doi":["10.3390/toxins15090584"]}}