{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang YL"],"funding":["MOST | National Natural Science Foundation of China"],"pagination":["e0099423"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10537586"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["89(9)"],"pubmed_abstract":["The anti-ultraviolet (UV) role of a Rad4-Rad23-Rad33 complex in budding yeast relies on nucleotide excision repair (NER), which is mechanistically distinct from photorepair of DNA lesions generated under solar UV irradiation but remains poorly known in filamentous fungi. Here, two nucleus-specific Rad4 paralogs (Rad4A and Rad4B) and nucleocytoplasmic shuttling Rad23 ortholog are functionally characterized by multiple analyses of their null mutants in <i>Metarhizium robertsii</i>, an entomopathogenic fungus lacking Rad33. Rad4A was proven to interact with Rad23 and contribute significantly more to conidial UVB resistance (90%) than Rad23 (65%). Despite no other biological function, Rad4A exhibited a very high activity in photoreactivation of UVB-impaired/inactivated conidia by 5-h light exp"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Divergent roles of Rad4 and Rad23 homologs in <i>Metarhizium robertsii</i>'s resistance to solar ultraviolet damage."],"pmcid":["PMC10537586"],"funding_grant_id":["32270086","31772218"],"pubmed_authors":["Peng H","Zhang YL","Zhang K","Ying SH","Feng MG"],"additional_accession":[]},"is_claimable":false,"name":"Divergent roles of Rad4 and Rad23 homologs in <i>Metarhizium robertsii</i>'s resistance to solar ultraviolet damage.","description":"The anti-ultraviolet (UV) role of a Rad4-Rad23-Rad33 complex in budding yeast relies on nucleotide excision repair (NER), which is mechanistically distinct from photorepair of DNA lesions generated under solar UV irradiation but remains poorly known in filamentous fungi. Here, two nucleus-specific Rad4 paralogs (Rad4A and Rad4B) and nucleocytoplasmic shuttling Rad23 ortholog are functionally characterized by multiple analyses of their null mutants in <i>Metarhizium robertsii</i>, an entomopathogenic fungus lacking Rad33. Rad4A was proven to interact with Rad23 and contribute significantly more to conidial UVB resistance (90%) than Rad23 (65%). Despite no other biological function, Rad4A exhibited a very high activity in photoreactivation of UVB-impaired/inactivated conidia by 5-h light exp","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2026-05-13T14:38:03.767Z","creation":"2025-02-18T23:48:30.52Z"},"accession":"S-EPMC10537586","cross_references":{"pubmed":["37655890"],"doi":["10.1128/aem.00994-23"]}}