biostudies-literatureDas SSNIDDK NIH HHSNHGRI NIH HHS1788-1804https://www.ebi.ac.uk/biostudies/studies/S-EPMC10538422biostudies-literatureUnknown43(10)<h4>Background</h4>Adipocytes are crucial regulators of cardiovascular health. However, not much is known about gene expression profiles of adipocytes residing in nonfat cardiovascular tissues, their genetic regulation, and contribution to coronary artery disease. Here, we investigated whether and how the gene expression profiles of adipocytes in the subcutaneous adipose tissue differ from adipocytes residing in the heart.<h4>Methods</h4>We used single-nucleus RNA-sequencing data sets of subcutaneous adipose tissue and heart and performed in-depth analysis of tissue-resident adipocytes and their cell-cell interactions.<h4>Results</h4>We first discovered tissue-specific features of tissue-resident adipocytes, identified functional pathways involved in their tissue specificity, and found genArteriosclerosis, thrombosis, and vascular biologyCross-Tissue Single-Nucleus RNA Sequencing Discovers Tissue-Resident Adipocytes Involved in Propanoate Metabolism in the Human Heart.PMC10538422R01 HG010505R01 DK132775Pietilainen KHKar AAlvarez MLee SHTPajukanta PRajkumar SDas SSfalseCross-Tissue Single-Nucleus RNA Sequencing Discovers Tissue-Resident Adipocytes Involved in Propanoate Metabolism in the Human Heart.<h4>Background</h4>Adipocytes are crucial regulators of cardiovascular health. However, not much is known about gene expression profiles of adipocytes residing in nonfat cardiovascular tissues, their genetic regulation, and contribution to coronary artery disease. Here, we investigated whether and how the gene expression profiles of adipocytes in the subcutaneous adipose tissue differ from adipocytes residing in the heart.<h4>Methods</h4>We used single-nucleus RNA-sequencing data sets of subcutaneous adipose tissue and heart and performed in-depth analysis of tissue-resident adipocytes and their cell-cell interactions.<h4>Results</h4>We first discovered tissue-specific features of tissue-resident adipocytes, identified functional pathways involved in their tissue specificity, and found gen2023-01-01T00:00:00Z2023 Oct2026-06-01T10:08:17.202Z2025-04-04T08:22:23.289ZS-EPMC105384223740952810.1161/ATVBAHA.123.31935810.1161/atvbaha.123.319358