{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sokol-Borrelli SL"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","NIAID NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","NINDS NIH HHS","NIGMS NIH HHS","U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development"],"pagination":["6078"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10539341"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Identification of regulators of Toxoplasma gondii bradyzoite development and cyst formation is the most direct way to address the importance of parasite development in long-term persistence and reactivation of this parasite. Here we show that a T. gondii gene (named Regulator of Cystogenesis 1; ROCY1) is sufficient for T. gondii bradyzoite formation in vitro and in vivo. ROCY1 encodes an RNA binding protein that has a preference for 3' regulatory regions of hundreds of T. gondii transcripts, and its RNA-binding domains are required to mediate bradyzoite development. Female mice infected with ΔROCY1 parasites have reduced (>90%) cyst burden. While viable parasites can be cultivated from brain tissue for up to 6 months post-infection, chronic brain-resident ΔROCY1 parasites have reduced oral"],"journal":["Nature communications"],"pubmed_title":["A transcriptional network required for bradyzoite development in Toxoplasma gondii is dispensable for recrudescent disease."],"pmcid":["PMC10539341"],"funding_grant_id":["F31 AI167594","R01AI120846","R01AI116855","T32 GM133353","F31NS118865","R21 AI167662","R01AI124723","F31 NS118865","R01 AI120846","F31AI167594","R21AI167662","F31 AI140529","R01 AI124723","R01 AI116855","T32GM133353","F31AI140529","P20 GM125498"],"pubmed_authors":["Cabo LF","Lodoen MB","Sullivan WJ","Holmes MJ","Reilly SM","Alrubaye HS","Sokol-Borrelli SL","Boyle JP","Sharp KG","Martorelli Di Genova B","Orchanian SB","Massmann MD"],"additional_accession":[]},"is_claimable":false,"name":"A transcriptional network required for bradyzoite development in Toxoplasma gondii is dispensable for recrudescent disease.","description":"Identification of regulators of Toxoplasma gondii bradyzoite development and cyst formation is the most direct way to address the importance of parasite development in long-term persistence and reactivation of this parasite. Here we show that a T. gondii gene (named Regulator of Cystogenesis 1; ROCY1) is sufficient for T. gondii bradyzoite formation in vitro and in vivo. ROCY1 encodes an RNA binding protein that has a preference for 3' regulatory regions of hundreds of T. gondii transcripts, and its RNA-binding domains are required to mediate bradyzoite development. Female mice infected with ΔROCY1 parasites have reduced (>90%) cyst burden. While viable parasites can be cultivated from brain tissue for up to 6 months post-infection, chronic brain-resident ΔROCY1 parasites have reduced oral","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2026-07-14T18:49:36.826Z","creation":"2025-04-04T08:24:11.227Z"},"accession":"S-EPMC10539341","cross_references":{"pubmed":["37770433"],"doi":["10.1038/s41467-023-40948-w"]}}