{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["19(15)"],"submitter":["Lei WL"],"pubmed_abstract":["Alternative splicing (AS) plays significant roles in a multitude of fundamental biological activities. AS is prevalent in the testis, but the regulations of AS in spermatogenesis is only little explored. Here, we report that Serine/arginine-rich splicing factor 1 (SRSF1) plays critical roles in alternative splicing and male reproduction. Male germ cell-specific deletion of <i>Srsf1</i> led to complete infertility by affecting spermatogenesis. Mechanistically, by combining RNA-seq data with LACE-seq data, we showed that SRSF1 affected the AS of <i>Stra8</i> in a direct manner and <i>Dazl</i>, <i>Dmc1</i>, <i>Mre11a</i>, <i>Syce2</i> and <i>Rif1</i> in an indirect manner. Our findings demonstrate that SRSF1 has crucial functions in spermatogenesis and male fertility by regulating alternative"],"journal":["International journal of biological sciences"],"pagination":["4883-4897"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10539708"],"repository":["biostudies-literature"],"pubmed_title":["SRSF1-mediated alternative splicing is required for spermatogenesis."],"pmcid":["PMC10539708"],"pubmed_authors":["Wang ZB","Lei WL","Du Z","Han Z","Su R","Meng TG","Ning Y","Sun QY","Sun F","Schatten H","Qian WP","Liu C","Li YY","Hou G"],"additional_accession":[]},"is_claimable":false,"name":"SRSF1-mediated alternative splicing is required for spermatogenesis.","description":"Alternative splicing (AS) plays significant roles in a multitude of fundamental biological activities. AS is prevalent in the testis, but the regulations of AS in spermatogenesis is only little explored. Here, we report that Serine/arginine-rich splicing factor 1 (SRSF1) plays critical roles in alternative splicing and male reproduction. Male germ cell-specific deletion of <i>Srsf1</i> led to complete infertility by affecting spermatogenesis. Mechanistically, by combining RNA-seq data with LACE-seq data, we showed that SRSF1 affected the AS of <i>Stra8</i> in a direct manner and <i>Dazl</i>, <i>Dmc1</i>, <i>Mre11a</i>, <i>Syce2</i> and <i>Rif1</i> in an indirect manner. Our findings demonstrate that SRSF1 has crucial functions in spermatogenesis and male fertility by regulating alternative","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-04-29T03:19:25.588Z","creation":"2025-04-05T15:23:34.761Z"},"accession":"S-EPMC10539708","cross_references":{"pubmed":["37781512"],"doi":["10.7150/ijbs.83474"]}}