biostudies-literatureUnknown19(15)Lei WLAlternative splicing (AS) plays significant roles in a multitude of fundamental biological activities. AS is prevalent in the testis, but the regulations of AS in spermatogenesis is only little explored. Here, we report that Serine/arginine-rich splicing factor 1 (SRSF1) plays critical roles in alternative splicing and male reproduction. Male germ cell-specific deletion of Srsf1 led to complete infertility by affecting spermatogenesis. Mechanistically, by combining RNA-seq data with LACE-seq data, we showed that SRSF1 affected the AS of Stra8 in a direct manner and Dazl, Dmc1, Mre11a, Syce2 and Rif1 in an indirect manner. Our findings demonstrate that SRSF1 has crucial functions in spermatogenesis and male fertility by regulating alternativeInternational journal of biological sciences4883-4897https://www.ebi.ac.uk/biostudies/studies/S-EPMC10539708biostudies-literatureSRSF1-mediated alternative splicing is required for spermatogenesis.PMC10539708Wang ZBLei WLDu ZHan ZSu RMeng TGNing YSun QYSun FSchatten HQian WPLiu CLi YYHou GfalseSRSF1-mediated alternative splicing is required for spermatogenesis.Alternative splicing (AS) plays significant roles in a multitude of fundamental biological activities. AS is prevalent in the testis, but the regulations of AS in spermatogenesis is only little explored. Here, we report that Serine/arginine-rich splicing factor 1 (SRSF1) plays critical roles in alternative splicing and male reproduction. Male germ cell-specific deletion of Srsf1 led to complete infertility by affecting spermatogenesis. Mechanistically, by combining RNA-seq data with LACE-seq data, we showed that SRSF1 affected the AS of Stra8 in a direct manner and Dazl, Dmc1, Mre11a, Syce2 and Rif1 in an indirect manner. Our findings demonstrate that SRSF1 has crucial functions in spermatogenesis and male fertility by regulating alternative2023-01-01T00:00:00Z20232026-04-29T03:19:25.588Z2025-04-05T15:23:34.761ZS-EPMC105397083778151210.7150/ijbs.83474