{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liang Z"],"funding":["General Program of the National Natural Science Foundation of China","National Key R&amp;D Program of China","Beijing Municipal Science and Technology Project","Changping laboratory"],"pagination":["e2261566"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10540657"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(2)"],"pubmed_abstract":["<b>ABSTRACT</b>The global outbreak of COVID-19 has caused a severe threat to human health; therefore, simple, high-throughput neutralization assays are desirable for developing vaccines and drugs against COVID-19. In this study, a high-titre SARS-CoV-2 pseudovirus was successfully packaged by truncating the C-terminus of the SARS-CoV-2 spike protein by 21 amino acids and infecting 293 T cells that had been stably transfected with the angiotensin-converting enzyme 2 (ACE2) receptor and furin (named AF cells), to establish a simple, high-throughput, and automated 384-well plate neutralization assay. The method was optimized for cell amount, virus inoculation, incubation time, and detection time. The automated assay showed good sensitivity, accuracy, reproducibility, Z' factor, and a good cor"],"journal":["Emerging microbes & infections"],"pubmed_title":["Development of an automated, high-throughput SARS-CoV-2 neutralization assay based on a pseudotyped virus using a vesicular stomatitis virus (VSV) vector."],"pmcid":["PMC10540657"],"funding_grant_id":["82172244 &amp; 32070678","Z211100002521018","2021YFC0863300","2021YFC2302500","2021YFC2301700"],"pubmed_authors":["Wu J","Zhao C","Liang Z","Nie J","Liu S","Zhang L","Li T","Huang W","Tong J","Qin H","Wang Y","Lu Q","Wu X","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"Development of an automated, high-throughput SARS-CoV-2 neutralization assay based on a pseudotyped virus using a vesicular stomatitis virus (VSV) vector.","description":"<b>ABSTRACT</b>The global outbreak of COVID-19 has caused a severe threat to human health; therefore, simple, high-throughput neutralization assays are desirable for developing vaccines and drugs against COVID-19. In this study, a high-titre SARS-CoV-2 pseudovirus was successfully packaged by truncating the C-terminus of the SARS-CoV-2 spike protein by 21 amino acids and infecting 293 T cells that had been stably transfected with the angiotensin-converting enzyme 2 (ACE2) receptor and furin (named AF cells), to establish a simple, high-throughput, and automated 384-well plate neutralization assay. The method was optimized for cell amount, virus inoculation, incubation time, and detection time. The automated assay showed good sensitivity, accuracy, reproducibility, Z' factor, and a good cor","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2026-05-29T01:58:01.725Z","creation":"2025-02-19T00:01:06.723Z"},"accession":"S-EPMC10540657","cross_references":{"pubmed":["37727107"],"doi":["10.1080/22221751.2023.2261566"]}}