{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(40)"],"submitter":["Bui CV"],"pubmed_abstract":["Several genetically encoded sensors have been developed to study live cell NADPH/NADP<sup>+</sup> dynamics, but their use has been predominantly in vitro. Here, we developed an in vivo assay using the Apollo-NADP<sup>+</sup> sensor and microfluidic devices to measure endogenous NADPH/NADP<sup>+</sup> dynamics in the pancreatic β cells of live zebrafish embryos. Flux through the pentose phosphate pathway, the main source of NADPH in many cell types, has been reported to be low in β cells. Thus, it is unclear how these cells compensate to meet NADPH demands. Using our assay, we show that pyruvate cycling is the main source of NADP<sup>+</sup> reduction in β cells, with contributions from folate cycling after acute electrical activation. INS1E β cells also showed a stress-induced increase in folate cycling and further suggested that this cycling requires both increased glycolytic intermediates and cytosolic NAD<sup>+</sup>. Overall, we show in vivo application of the Apollo-NADP<sup>+</sup> sensor and reveal that β cells are capable of adapting NADPH/NADP<sup>+</sup> redox during stress."],"journal":["Science advances"],"pagination":["eadi8317"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10550227"],"repository":["biostudies-literature"],"pubmed_title":["Apollo-NADP<sup>+</sup> reveals in vivo adaptation of NADPH/NADP<sup>+</sup> metabolism in electrically activated pancreatic β cells."],"pmcid":["PMC10550227"],"pubmed_authors":["Boswell CW","Ciruna B","Bui CV","Rocheleau JV"],"additional_accession":[]},"is_claimable":false,"name":"Apollo-NADP<sup>+</sup> reveals in vivo adaptation of NADPH/NADP<sup>+</sup> metabolism in electrically activated pancreatic β cells.","description":"Several genetically encoded sensors have been developed to study live cell NADPH/NADP<sup>+</sup> dynamics, but their use has been predominantly in vitro. Here, we developed an in vivo assay using the Apollo-NADP<sup>+</sup> sensor and microfluidic devices to measure endogenous NADPH/NADP<sup>+</sup> dynamics in the pancreatic β cells of live zebrafish embryos. Flux through the pentose phosphate pathway, the main source of NADPH in many cell types, has been reported to be low in β cells. Thus, it is unclear how these cells compensate to meet NADPH demands. Using our assay, we show that pyruvate cycling is the main source of NADP<sup>+</sup> reduction in β cells, with contributions from folate cycling after acute electrical activation. INS1E β cells also showed a stress-induced increase in folate cycling and further suggested that this cycling requires both increased glycolytic intermediates and cytosolic NAD<sup>+</sup>. Overall, we show in vivo application of the Apollo-NADP<sup>+</sup> sensor and reveal that β cells are capable of adapting NADPH/NADP<sup>+</sup> redox during stress.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2025-04-18T13:25:05.347Z","creation":"2025-04-04T08:46:11.499Z"},"accession":"S-EPMC10550227","cross_references":{"pubmed":["37792934"],"doi":["10.1126/sciadv.adi8317"]}}