{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(4)"],"submitter":["Parisi B"],"pubmed_abstract":["The C2C12 cell line represents a simple in vitro model for cell differentiation. Here, we present a flow-cytometry-based pipeline to quantitate C2C12 cell differentiation based on myosin heavy-chain marker expression. We describe steps for cell seeding, transfection, drug treatment, differentiation, and labeling. We then detail procedures for flow cytometry acquisition and introduce the R script FlowFate for automated analysis, including the study of dose-dependent effects of GFP-tagged genes on differentiation. For complete details on the use and execution of this protocol, please refer to Chippalkatti et al. (2023).<sup>1</sup>."],"journal":["STAR protocols"],"pagination":["102637"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10568640"],"repository":["biostudies-literature"],"pubmed_title":["A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiation."],"pmcid":["PMC10568640"],"pubmed_authors":["Parisi B","Sunnen M","Abankwa DK","Chippalkatti R"],"additional_accession":[]},"is_claimable":false,"name":"A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiation.","description":"The C2C12 cell line represents a simple in vitro model for cell differentiation. Here, we present a flow-cytometry-based pipeline to quantitate C2C12 cell differentiation based on myosin heavy-chain marker expression. We describe steps for cell seeding, transfection, drug treatment, differentiation, and labeling. We then detail procedures for flow cytometry acquisition and introduce the R script FlowFate for automated analysis, including the study of dose-dependent effects of GFP-tagged genes on differentiation. For complete details on the use and execution of this protocol, please refer to Chippalkatti et al. (2023).<sup>1</sup>.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2025-04-22T11:03:18.84Z","creation":"2025-04-05T23:47:31.556Z"},"accession":"S-EPMC10568640","cross_references":{"pubmed":["37819762"],"doi":["10.1016/j.xpro.2023.102637"]}}