{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wellington D"],"funding":["Medical Research Council"],"pagination":["e20076"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10570596"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(10)"],"pubmed_abstract":["Mutations within viral epitopes can result in escape from T cells, but the contribution of mutations in flanking regions of epitopes in SARS-CoV-2 has not been investigated. Focusing on two SARS-CoV-2 nucleoprotein CD8<sup>+</sup> epitopes, we investigated the contribution of these flanking mutations to proteasomal processing and T cell activation. We found decreased NP<sub>9-17</sub>-B*27:05 CD8<sup>+</sup> T cell responses to the NP-Q7K mutation, likely due to a lack of efficient epitope production by the proteasome, suggesting immune escape caused by this mutation. In contrast, NP-P6L and NP-D103 N/Y mutations flanking the NP<sub>9-17</sub>-B*27:05 and NP<sub>105-113</sub>-B*07:02 epitopes, respectively, increased CD8<sup>+</sup> T cell responses associated with enhanced epitope product"],"journal":["Heliyon"],"pubmed_title":["SARS-CoV-2 mutations affect antigen processing by the proteasome to alter CD8&lt;sup&gt;+&lt;/sup&gt; T cell responses."],"pmcid":["PMC10570596"],"funding_grant_id":["G0501068"],"pubmed_authors":["Peng Y","Dong T","Fischer R","Davis S","Heilig R","Kessler BM","Yao X","Yin Z","Dong D","Wellington D","Antoun E","Yu Z","Hublitz P","Beveridge R","Felce SL","Liu G"],"additional_accession":[]},"is_claimable":false,"name":"SARS-CoV-2 mutations affect antigen processing by the proteasome to alter CD8&lt;sup&gt;+&lt;/sup&gt; T cell responses.","description":"Mutations within viral epitopes can result in escape from T cells, but the contribution of mutations in flanking regions of epitopes in SARS-CoV-2 has not been investigated. Focusing on two SARS-CoV-2 nucleoprotein CD8<sup>+</sup> epitopes, we investigated the contribution of these flanking mutations to proteasomal processing and T cell activation. We found decreased NP<sub>9-17</sub>-B*27:05 CD8<sup>+</sup> T cell responses to the NP-Q7K mutation, likely due to a lack of efficient epitope production by the proteasome, suggesting immune escape caused by this mutation. In contrast, NP-P6L and NP-D103 N/Y mutations flanking the NP<sub>9-17</sub>-B*27:05 and NP<sub>105-113</sub>-B*07:02 epitopes, respectively, increased CD8<sup>+</sup> T cell responses associated with enhanced epitope product","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2026-05-29T06:11:54.366Z","creation":"2025-04-06T01:59:41.242Z"},"accession":"S-EPMC10570596","cross_references":{"pubmed":["37842619"],"doi":["10.1016/j.heliyon.2023.e20076"]}}