biostudies-literatureYe MSichuan Provincial Administration of Traditional Chinese Medicine17385https://www.ebi.ac.uk/biostudies/studies/S-EPMC10575897biostudies-literatureUnknown13(1)To investigate the potential mechanism of Er-Xian decoction (EXD) in treating aplastic anemia (AA), the active components of EXD were screened by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), and the targets of the components were predicted by the Swiss Target Prediction database. AA targets were collected from the GeneCards, OMIM, DisGeNET, PharmGKB, DrugBank, and TTD databases, the intersection of AA targets and EXD targets was calculated, and an herb-component-target network was constructed by Cytoscape 3.7.2 software. The STRING database was used for protein‒protein interaction (PPI) analysis, and Cytoscape 3.7.2 software was used to construct a PPI network and perform topology analysis. The core targets were imported into the DAVID databScientific reportsNetwork pharmacology and experimental verification of the potential mechanism of Er-Xian decoction in aplastic anemia.PMC105758972021MS205Ye MYang HYang YRen JChen WGao ZLiu GfalseNetwork pharmacology and experimental verification of the potential mechanism of Er-Xian decoction in aplastic anemia.To investigate the potential mechanism of Er-Xian decoction (EXD) in treating aplastic anemia (AA), the active components of EXD were screened by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), and the targets of the components were predicted by the Swiss Target Prediction database. AA targets were collected from the GeneCards, OMIM, DisGeNET, PharmGKB, DrugBank, and TTD databases, the intersection of AA targets and EXD targets was calculated, and an herb-component-target network was constructed by Cytoscape 3.7.2 software. The STRING database was used for protein‒protein interaction (PPI) analysis, and Cytoscape 3.7.2 software was used to construct a PPI network and perform topology analysis. The core targets were imported into the DAVID datab2023-01-01T00:00:00Z2023 Oct2025-04-04T21:01:59.514Z2025-04-04T21:01:59.514ZS-EPMC105758973783336310.1038/s41598-023-44672-9