biostudies-literatureSchmid AMBioTechMed-Graz FlagshipAustrian Science Fund FWFEuropean UnionThe Government of Lower AustriaOeAD WTZOeAD-GmbH297https://www.ebi.ac.uk/biostudies/studies/S-EPMC10588034biostudies-literatureUnknown21(1)<h4>Background</h4>E. coli O83 (Colinfant Newborn) is a Gram-negative (G-) probiotic bacterium used in the clinic. When administered orally, it reduces allergic sensitisation but not allergic asthma. Intranasal administration offers a non-invasive and convenient delivery method. This route bypasses the gastrointestinal tract and provides direct access to the airways, which are the target of asthma prevention. G- bacteria such as E. coli O83 release outer membrane vesicles (OMVs) to communicate with the environment. Here we investigate whether intranasally administered E. coli O83 OMVs (EcO83-OMVs) can reduce allergic airway inflammation in mice.<h4>Methods</h4>EcO83-OMVs were isolated by ultracentrifugation and characterised their number, morphology (shape and size), composition (proteins Cell communication and signaling : CCSExtracellular vesicles of the probiotic bacteria E. coli O83 activate innate immunity and prevent allergy in mice.PMC10588034RS 08/2022DK W1248-B30Project SecretomeCZ 06/2020P 33073Danube Allergy Research Cluster101066450P 34867CZ 07/2023Schild SSchabussova IKerekes DInic-Kanada AWysmolek MThaler MSchmidt KWiedermann UKohl PHaunstetter MPalmfeldt JNejsum PWeinmayer TGeissler NWhitehead BSindelar MHrdy JRazim ASchmid AMBrazhnikov GKrcmarova EfalseExtracellular vesicles of the probiotic bacteria E. coli O83 activate innate immunity and prevent allergy in mice.<h4>Background</h4>E. coli O83 (Colinfant Newborn) is a Gram-negative (G-) probiotic bacterium used in the clinic. When administered orally, it reduces allergic sensitisation but not allergic asthma. Intranasal administration offers a non-invasive and convenient delivery method. This route bypasses the gastrointestinal tract and provides direct access to the airways, which are the target of asthma prevention. G- bacteria such as E. coli O83 release outer membrane vesicles (OMVs) to communicate with the environment. Here we investigate whether intranasally administered E. coli O83 OMVs (EcO83-OMVs) can reduce allergic airway inflammation in mice.<h4>Methods</h4>EcO83-OMVs were isolated by ultracentrifugation and characterised their number, morphology (shape and size), composition (proteins 2023-01-01T00:00:00Z2023 Oct2026-05-28T11:14:31.52Z2026-05-13T14:22:22.345ZS-EPMC105880343786421110.1186/s12964-023-01329-4