biostudies-literatureNimerovsky EDeutsche ForschungsgemeinschaftMax-Planck-Gesellschaft2763-2771https://www.ebi.ac.uk/biostudies/studies/S-EPMC10598565biostudies-literatureUnknown3(10)Inspired by the recently proposed transverse mixing optimal control pulses (TROP) approach for improving signal in multidimensional magic-angle spinning (MAS) NMR experiments, we present simplified preservation of equivalent pathways spectroscopy (SPEPS). It transfers both transverse components of magnetization that occur during indirect evolutions, theoretically enabling a √2 improvement in sensitivity for each such dimension. We compare SPEPS transfer with TROP and cross-polarization (CP) using membrane protein and fibril samples at MAS of 55 and 100 kHz. In three-dimensional (3D) (H)CANH spectra, SPEPS outperformed TROP and CP by factors of on average 1.16 and 1.69, respectively, for the membrane protein, but only a marginal improvement of 1.09 was observed for the fibril. These differeJACS AuSimplified Preservation of Equivalent Pathways Spectroscopy.PMC10598565AN1316Andreas LBVarkey ACBecker SKim MNimerovsky EfalseSimplified Preservation of Equivalent Pathways Spectroscopy.Inspired by the recently proposed transverse mixing optimal control pulses (TROP) approach for improving signal in multidimensional magic-angle spinning (MAS) NMR experiments, we present simplified preservation of equivalent pathways spectroscopy (SPEPS). It transfers both transverse components of magnetization that occur during indirect evolutions, theoretically enabling a √2 improvement in sensitivity for each such dimension. We compare SPEPS transfer with TROP and cross-polarization (CP) using membrane protein and fibril samples at MAS of 55 and 100 kHz. In three-dimensional (3D) (H)CANH spectra, SPEPS outperformed TROP and CP by factors of on average 1.16 and 1.69, respectively, for the membrane protein, but only a marginal improvement of 1.09 was observed for the fibril. These differe2023-01-01T00:00:00Z2023 Oct2026-05-29T07:34:18.238Z2026-05-16T03:07:34.458ZS-EPMC105985653788557710.1021/jacsau.3c00312