biostudies-literatureMothersole RGNatural Sciences and Engineering Research Council of CanadaStrategic Environmental Research and Development Program39437-39446https://www.ebi.ac.uk/biostudies/studies/S-EPMC10601079biostudies-literatureUnknown8(42)Perfluoroalkyl and polyfluoroalkyl substances (PFAS) make up a group of anthropogenic chemicals with a myriad of applications. However, some PFAS have been shown to negatively impact human health and the environment, leading to increased regulation, with some countries making efforts to phase out their use. PFAS fate in the environment is driven by physical, chemical, and biological processes, with microbial communities in matrices such as soil and sewage sludge being known to generate a range of low-molecular-weight PFAS metabolites. Proposed metabolic intermediates for both mixed and pure microbial cultures include fluorinated carboxylates that may be activated by CoA prior to β-oxidation and defluorination, although thus far, no PFAS-CoA adducts have been reported. Herein, we expressed ACS omegaFormation of CoA Adducts of Short-Chain Fluorinated Carboxylates Catalyzed by Acyl-CoA Synthetase from <i>Gordonia</i> sp. Strain NB4-1Y.PMC10601079ER-1023Wynne FTLiu JMothersole MKVan Hamme JDMothersole RGRota GfalseFormation of CoA Adducts of Short-Chain Fluorinated Carboxylates Catalyzed by Acyl-CoA Synthetase from <i>Gordonia</i> sp. Strain NB4-1Y.Perfluoroalkyl and polyfluoroalkyl substances (PFAS) make up a group of anthropogenic chemicals with a myriad of applications. However, some PFAS have been shown to negatively impact human health and the environment, leading to increased regulation, with some countries making efforts to phase out their use. PFAS fate in the environment is driven by physical, chemical, and biological processes, with microbial communities in matrices such as soil and sewage sludge being known to generate a range of low-molecular-weight PFAS metabolites. Proposed metabolic intermediates for both mixed and pure microbial cultures include fluorinated carboxylates that may be activated by CoA prior to β-oxidation and defluorination, although thus far, no PFAS-CoA adducts have been reported. Herein, we expressed 2023-01-01T00:00:00Z2023 Oct2026-04-29T02:38:27.194Z2025-02-19T01:20:24.145ZS-EPMC106010793790152810.1021/acsomega.3c05147