biostudies-literatureLim JSMinistry of EducationKorea Food Research Institute (KFRI) funded by the Ministry of ScienceNational Research Foundation of Korea (NRF)Ministry of ScienceKorea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative ProgramNational Research Foundation of Korea1792https://www.ebi.ac.uk/biostudies/studies/S-EPMC10604099biostudies-literatureUnknown12(10)<i>Ehretia tinifolia</i> (<i>E. tinifolia</i>) L., an evergreen tree with substantial biological activity, including antioxidant and anti-inflammatory effects, has been used in many herbal and traditional medicines. To elucidate its antioxidant and anti-inflammatory activity and the underlying mechanisms, we applied a methanol extract of <i>E. tinifolia</i> (ETME) to lipopolysaccharide (LPS)-stimulated mouse immortalized Kupffer cells. ETME suppressed the LPS-induced increase in nitric oxide, a mediator for oxidative stress and inflammation, and restored LPS-mediated depletion of total glutathione level by stabilizing antioxidative nuclear factor erythroid 2-related factor 2 (Nrf2) and the subsequent increase in heme oxygenase-1 levels. Furthermore, ETME inhibited the LPS-induced productioAntioxidants (Basel, Switzerland)Inhibitory Effects of <i>Ehretia tinifolia</i> Extract on the Excessive Oxidative and Inflammatory Responses in Lipopolysaccharide-Stimulated Mouse Kupffer Cells.PMC10604099Main Research Program E0210300NRF-2020R1C1C1007261E0210300NRF-2022R1I1A1A01056975KGM5242322Cho YCLim JSLee DYYun HLee SHBach TTCho NChoi JUYoo GLee KYPark SJfalseInhibitory Effects of <i>Ehretia tinifolia</i> Extract on the Excessive Oxidative and Inflammatory Responses in Lipopolysaccharide-Stimulated Mouse Kupffer Cells.<i>Ehretia tinifolia</i> (<i>E. tinifolia</i>) L., an evergreen tree with substantial biological activity, including antioxidant and anti-inflammatory effects, has been used in many herbal and traditional medicines. To elucidate its antioxidant and anti-inflammatory activity and the underlying mechanisms, we applied a methanol extract of <i>E. tinifolia</i> (ETME) to lipopolysaccharide (LPS)-stimulated mouse immortalized Kupffer cells. ETME suppressed the LPS-induced increase in nitric oxide, a mediator for oxidative stress and inflammation, and restored LPS-mediated depletion of total glutathione level by stabilizing antioxidative nuclear factor erythroid 2-related factor 2 (Nrf2) and the subsequent increase in heme oxygenase-1 levels. Furthermore, ETME inhibited the LPS-induced productio2023-01-01T00:00:00Z2023 Sep2026-06-22T03:21:57.989Z2025-04-04T10:07:01.89ZS-EPMC106040993789187210.3390/antiox12101792