{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang M"],"funding":["China National Natural Science Foundation Projects for Youth","Special projects for capacity building in scientific and technological innovation of the Beijing Academy of Agriculture and Forestry","Key research and development projects of the National Forestry and Grassland Administration"],"pagination":["15304"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10607007"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(20)"],"pubmed_abstract":["Lily (<i>Lilium</i> spp.) is a popular ornamental plant. Traditional genetic transformation methods have low efficiency in lily, thus development of a high-efficiency genetic transformation system is important. In this study, a novel transient transformation method involving pollen magnetofection was established and optimized pollen viability, and exogenous gene expression in magnetofected pollen and that of different germplasm were assessed. The highest germination percentage of <i>Lilium regale</i> pollen was 85.73% in medium containing 100 g/L sucrose, 61.5 mg/L H<sub>3</sub>BO<sub>3</sub>, and 91.5 mg/L CaCl<sub>2</sub>. A 1:4 ratio of nanomagnetic beads to DNA plasmid and transformation time of 0.5 h realized the highest transformation efficiency (88.32%). The GFP activity in transfor"],"journal":["International journal of molecular sciences"],"pubmed_title":["A Modified Method for Transient Transformation via Pollen Magnetofection in <i>Lilium</i> Germplasm."],"pmcid":["PMC10607007"],"funding_grant_id":["32201606","GLM [2021] No. 72","KJCX20230203"],"pubmed_authors":["Jin G","Zhao C","Zhang X","Ma X","Zhang M","Du Y","Chen X","Xue J","Han D","Yang F"],"additional_accession":[]},"is_claimable":false,"name":"A Modified Method for Transient Transformation via Pollen Magnetofection in <i>Lilium</i> Germplasm.","description":"Lily (<i>Lilium</i> spp.) is a popular ornamental plant. Traditional genetic transformation methods have low efficiency in lily, thus development of a high-efficiency genetic transformation system is important. In this study, a novel transient transformation method involving pollen magnetofection was established and optimized pollen viability, and exogenous gene expression in magnetofected pollen and that of different germplasm were assessed. The highest germination percentage of <i>Lilium regale</i> pollen was 85.73% in medium containing 100 g/L sucrose, 61.5 mg/L H<sub>3</sub>BO<sub>3</sub>, and 91.5 mg/L CaCl<sub>2</sub>. A 1:4 ratio of nanomagnetic beads to DNA plasmid and transformation time of 0.5 h realized the highest transformation efficiency (88.32%). The GFP activity in transfor","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2025-06-01T02:30:48.24Z","creation":"2025-06-01T02:30:48.24Z"},"accession":"S-EPMC10607007","cross_references":{"pubmed":["37894985"],"doi":["10.3390/ijms242015304"]}}