{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yuan G"],"funding":["the National Transgenic Major Project of China","the National Natural Science Foundation of China"],"pagination":["803"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10607264"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(10)"],"pubmed_abstract":["Genetic engineering technology offers opportunities to improve many important agronomic traits in crops, including insect-resistance. However, genetically modified (GM) exogenous proteins in edible tissues of transgenic crops has become an issue of intense public concern. To advance the application of GM techniques in maize, a Cre/<i>lox</i>P-based strategy was developed for manipulating the transgenes in green tissues while locking them in non-green tissues. In the strategy, the site-specific excision can be used to switch on or off the expression of transgenes at specific tissues. In this work, two basic transgenic maize, named KEY, carrying the <i>Cre</i> gene, and LOCK, containing the <i>Vip3A</i> gene with a blocked element, were obtained based on their separate fusion gene cassettes."],"journal":["Insects"],"pubmed_title":["Engineered Expression of Vip3A in Green Tissues as a Feasible Approach for the Control of Insect Pests in Maize."],"pmcid":["PMC10607264"],"funding_grant_id":["32272177","2019ZX08010-002"],"pubmed_authors":["Zeng C","Pan G","Yang Y","Ma L","Du J","Zou C","Shen Y","Yuan G","Shi H"],"additional_accession":[]},"is_claimable":false,"name":"Engineered Expression of Vip3A in Green Tissues as a Feasible Approach for the Control of Insect Pests in Maize.","description":"Genetic engineering technology offers opportunities to improve many important agronomic traits in crops, including insect-resistance. However, genetically modified (GM) exogenous proteins in edible tissues of transgenic crops has become an issue of intense public concern. To advance the application of GM techniques in maize, a Cre/<i>lox</i>P-based strategy was developed for manipulating the transgenes in green tissues while locking them in non-green tissues. In the strategy, the site-specific excision can be used to switch on or off the expression of transgenes at specific tissues. In this work, two basic transgenic maize, named KEY, carrying the <i>Cre</i> gene, and LOCK, containing the <i>Vip3A</i> gene with a blocked element, were obtained based on their separate fusion gene cassettes.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2025-04-22T12:00:38.581Z","creation":"2025-04-06T00:09:11.016Z"},"accession":"S-EPMC10607264","cross_references":{"pubmed":["37887815"],"doi":["10.3390/insects14100803"]}}