biostudies-literatureYuan Gthe National Transgenic Major Project of Chinathe National Natural Science Foundation of China803https://www.ebi.ac.uk/biostudies/studies/S-EPMC10607264biostudies-literatureUnknown14(10)Genetic engineering technology offers opportunities to improve many important agronomic traits in crops, including insect-resistance. However, genetically modified (GM) exogenous proteins in edible tissues of transgenic crops has become an issue of intense public concern. To advance the application of GM techniques in maize, a Cre/<i>lox</i>P-based strategy was developed for manipulating the transgenes in green tissues while locking them in non-green tissues. In the strategy, the site-specific excision can be used to switch on or off the expression of transgenes at specific tissues. In this work, two basic transgenic maize, named KEY, carrying the <i>Cre</i> gene, and LOCK, containing the <i>Vip3A</i> gene with a blocked element, were obtained based on their separate fusion gene cassettes.InsectsEngineered Expression of Vip3A in Green Tissues as a Feasible Approach for the Control of Insect Pests in Maize.PMC10607264322721772019ZX08010-002Zeng CPan GYang YMa LDu JZou CShen YYuan GShi HfalseEngineered Expression of Vip3A in Green Tissues as a Feasible Approach for the Control of Insect Pests in Maize.Genetic engineering technology offers opportunities to improve many important agronomic traits in crops, including insect-resistance. However, genetically modified (GM) exogenous proteins in edible tissues of transgenic crops has become an issue of intense public concern. To advance the application of GM techniques in maize, a Cre/<i>lox</i>P-based strategy was developed for manipulating the transgenes in green tissues while locking them in non-green tissues. In the strategy, the site-specific excision can be used to switch on or off the expression of transgenes at specific tissues. In this work, two basic transgenic maize, named KEY, carrying the <i>Cre</i> gene, and LOCK, containing the <i>Vip3A</i> gene with a blocked element, were obtained based on their separate fusion gene cassettes.2023-01-01T00:00:00Z2023 Oct2025-04-22T12:00:38.581Z2025-04-06T00:09:11.016ZS-EPMC106072643788781510.3390/insects14100803