biostudies-literatureZaharieva AOperational Program Science and Education for Smart Growth 2014-2020 co-financed by the European Union through the European Structural and Investment Funds, and by the Bulgarian Ministry of Education and ScienceBulgarian National Science FundBulgarian Science FundEuropean Structural and Investment FundsBulgarian Ministry of Education and Science1099https://www.ebi.ac.uk/biostudies/studies/S-EPMC10609352biostudies-literatureUnknown13(10)<i>Nepeta nuda</i> L. is a medicinal plant enriched with secondary metabolites serving to attract pollinators and deter herbivores. Phenolics and iridoids of <i>N. nuda</i> have been extensively investigated because of their beneficial impacts on human health. This study explores the chemical profiles of in vitro shoots and wild-grown <i>N. nuda</i> plants (flowers and leaves) through metabolomic analysis utilizing gas chromatography and mass spectrometry (GC-MS). Initially, we examined the differences in the volatiles' composition in in vitro-cultivated shoots comparing them with flowers and leaves from plants growing in natural environment. The characteristic iridoid 4a-α,7-β,7a-α-nepetalactone was highly represented in shoots of in vitro plants and in flowers of plants from nature populMetabolitesUncovering the Interrelation between Metabolite Profiles and Bioactivity of In Vitro- and Wild-Grown Catmint (<i>Nepeta nuda</i> L.).PMC10609352KP-06-N56/9/12.11.2021BG05M2OP001-1.002-0012Zehirov GTsacheva IMishev KRusanov KMisic DMotyka VZaharieva APetrova DDobrev PIMantovska DRusanova MYordanova ZFilepova RVassileva VLacek JPaunov MChaneva GZhiponova MfalseUncovering the Interrelation between Metabolite Profiles and Bioactivity of In Vitro- and Wild-Grown Catmint (<i>Nepeta nuda</i> L.).<i>Nepeta nuda</i> L. is a medicinal plant enriched with secondary metabolites serving to attract pollinators and deter herbivores. Phenolics and iridoids of <i>N. nuda</i> have been extensively investigated because of their beneficial impacts on human health. This study explores the chemical profiles of in vitro shoots and wild-grown <i>N. nuda</i> plants (flowers and leaves) through metabolomic analysis utilizing gas chromatography and mass spectrometry (GC-MS). Initially, we examined the differences in the volatiles' composition in in vitro-cultivated shoots comparing them with flowers and leaves from plants growing in natural environment. The characteristic iridoid 4a-α,7-β,7a-α-nepetalactone was highly represented in shoots of in vitro plants and in flowers of plants from nature popul2023-01-01T00:00:00Z2023 Oct2026-05-13T14:45:03.498Z2025-04-06T00:07:29.477ZS-EPMC106093523788742410.3390/metabo13101099