biostudies-literatureMiranda KJBritish Council PhilippinesCommission on Higher Education2563https://www.ebi.ac.uk/biostudies/studies/S-EPMC10609385biostudies-literatureUnknown11(10)Genome mining and metabolomics have become valuable tools in natural products research to evaluate and identify potential new chemistry from bacteria. In the search for new compounds from the deep-sea organism, <i>Pseudomonas</i> sp. SST3, from the South Shetland Trough, Antarctica, a co-cultivation with a second deep-sea <i>Pseudomonas zhaodongensis</i> SST2, was undertaken to isolate pseudomonassin, a ribosomally synthesised and post-translationally modified peptide (RiPP) that belongs to a class of RiPP called lasso peptides. Pseudomonassin was identified using a genome-mining approach and isolated by means of mass spectrometric guided isolation. Extensive metabolomics analysis of the co-cultivation of <i>Pseudomonas</i> sp. SST3 and <i>P. zhaodongensis</i> SST2, <i>Pseudomonas</i> sp. MicroorganismsPseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3.PMC10609385CHED-British Council Newton Agham Fellowship GrantEdrada-Ebel RMiranda KJAtoum DEbel RJaspars MJaber SArjunan SfalsePseudomonassin, a New Bioactive Ribosomally Synthesised and Post-Translationally Modified Peptide from <i>Pseudomonas</i> sp. SST3.Genome mining and metabolomics have become valuable tools in natural products research to evaluate and identify potential new chemistry from bacteria. In the search for new compounds from the deep-sea organism, <i>Pseudomonas</i> sp. SST3, from the South Shetland Trough, Antarctica, a co-cultivation with a second deep-sea <i>Pseudomonas zhaodongensis</i> SST2, was undertaken to isolate pseudomonassin, a ribosomally synthesised and post-translationally modified peptide (RiPP) that belongs to a class of RiPP called lasso peptides. Pseudomonassin was identified using a genome-mining approach and isolated by means of mass spectrometric guided isolation. Extensive metabolomics analysis of the co-cultivation of <i>Pseudomonas</i> sp. SST3 and <i>P. zhaodongensis</i> SST2, <i>Pseudomonas</i> sp. 2023-01-01T00:00:00Z2023 Oct2025-04-25T17:26:42.131Z2025-04-06T05:09:57.177ZS-EPMC106093853789422110.3390/microorganisms11102563