biostudies-literatureZhou Zthe Cooperation Project of the Agricultural Science and Technology Program of Zhejiang Province, Chinathe research fund of the Zhejiang Academy of Agricultural Sciences3565https://www.ebi.ac.uk/biostudies/studies/S-EPMC10609702biostudies-literatureUnknown12(20)<i>Sanghuangporus</i>, a medicinal mushroom, has gained significant attention due to its beneficial properties. Phenolic acids are among the major bioactive compounds in <i>Sanghuangporus</i>, known for their antioxidant and anti-inflammatory activities. To precisely quantify the phenolic acid content, we developed a method utilizing ultra-high-performance liquid chromatography with triple quadrupole (UHPLC-QqQ). This study optimized the UHPLC-QqQ conditions to simultaneously separate and detect eight phenolic acids in <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai, including chlorogenic acid, <i>p</i>-coumaric acid, caffeic acid, cryptochlorogenic acid, protocatechuic acid, ferulic acid, sinapic acid, and syringic acid. The separation process utilized a ZORBAX Eclipse Plus C1Plants (Basel, Switzerland)Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilat) L.W. Zhou and Y.C. Dai.PMC106097022022SNJF04610417000121CF0401G/004Liang SZou XTeng YWang WFu LZhou ZfalseDetermination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of <i>Sanghuangporus baumii</i> (Pilat) L.W. Zhou and Y.C. Dai.<i>Sanghuangporus</i>, a medicinal mushroom, has gained significant attention due to its beneficial properties. Phenolic acids are among the major bioactive compounds in <i>Sanghuangporus</i>, known for their antioxidant and anti-inflammatory activities. To precisely quantify the phenolic acid content, we developed a method utilizing ultra-high-performance liquid chromatography with triple quadrupole (UHPLC-QqQ). This study optimized the UHPLC-QqQ conditions to simultaneously separate and detect eight phenolic acids in <i>Sanghuangporus baumii</i> (Pilát) L.W. Zhou and Y.C. Dai, including chlorogenic acid, <i>p</i>-coumaric acid, caffeic acid, cryptochlorogenic acid, protocatechuic acid, ferulic acid, sinapic acid, and syringic acid. The separation process utilized a ZORBAX Eclipse Plus C12023-01-01T00:00:00Z2023 Oct2025-04-04T10:07:17.233Z2025-04-04T10:07:17.233ZS-EPMC106097023789602710.3390/plants12203565