{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Charles S"],"funding":["National Institute of Neurological Disorders and Stroke","Simons Foundation","NIA NIH HHS","Division of Biological Infrastructure","NINDS NIH HHS","Division of Mathematical Sciences","National Institute of General Medical Sciences","NIGMS NIH HHS","National Institute on Aging","Division of Chemical, Bioengineering, Environmental, and Transport Systems"],"pagination":["1369-1376"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10619480"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["93(3)"],"pubmed_abstract":["Recent development in fluorescence-based molecular tools has contributed significantly to developmental studies, including embryogenesis. Many of these tools rely on multiple steps of sample manipulation, so obtaining large sample sizes presents a major challenge as it can be labor-intensive and time-consuming. However, large sample sizes are required to uncover critical aspects of embryogenesis, for example, subtle phenotypic differences or gene expression dynamics. This problem is particularly relevant for single-molecule fluorescence in situ hybridization (smFISH) studies in <i>Caenorhabditis elegans</i> embryogenesis. Microfluidics can help address this issue by allowing a large number of samples and parallelization of experiments. However, performing efficient reagent exchange on chip"],"journal":["Analytical chemistry"],"pubmed_title":["High-Temporal-Resolution smFISH Method for Gene Expression Studies in <i>Caenorhabditis elegans</i> Embryos."],"pmcid":["PMC10619480"],"funding_grant_id":["R01 AG056436","1707401","R01 NS115484","R35 GM119744","R01 GM088333","1764406","R01AG056436","R21NS117066","R35GM119744","R21 NS117066","R01GM088333","R01 NS096581","R01NS115484","R01NS096581","0939511"],"pubmed_authors":["Lu H","Paaby AB","Aubry G","Chou HT","Charles S"],"additional_accession":[]},"is_claimable":false,"name":"High-Temporal-Resolution smFISH Method for Gene Expression Studies in <i>Caenorhabditis elegans</i> Embryos.","description":"Recent development in fluorescence-based molecular tools has contributed significantly to developmental studies, including embryogenesis. Many of these tools rely on multiple steps of sample manipulation, so obtaining large sample sizes presents a major challenge as it can be labor-intensive and time-consuming. However, large sample sizes are required to uncover critical aspects of embryogenesis, for example, subtle phenotypic differences or gene expression dynamics. This problem is particularly relevant for single-molecule fluorescence in situ hybridization (smFISH) studies in <i>Caenorhabditis elegans</i> embryogenesis. Microfluidics can help address this issue by allowing a large number of samples and parallelization of experiments. However, performing efficient reagent exchange on chip","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2026-06-06T21:13:09.494Z","creation":"2025-04-04T14:35:49.421Z"},"accession":"S-EPMC10619480","cross_references":{"pubmed":["33355449"],"doi":["10.1021/acs.analchem.0c02966"]}}