biostudies-literatureCharles SNational Institute of Neurological Disorders and StrokeSimons FoundationNIA NIH HHSDivision of Biological InfrastructureNINDS NIH HHSDivision of Mathematical SciencesNational Institute of General Medical SciencesNIGMS NIH HHSNational Institute on AgingDivision of Chemical, Bioengineering, Environmental, and Transport Systems1369-1376https://www.ebi.ac.uk/biostudies/studies/S-EPMC10619480biostudies-literatureUnknown93(3)Recent development in fluorescence-based molecular tools has contributed significantly to developmental studies, including embryogenesis. Many of these tools rely on multiple steps of sample manipulation, so obtaining large sample sizes presents a major challenge as it can be labor-intensive and time-consuming. However, large sample sizes are required to uncover critical aspects of embryogenesis, for example, subtle phenotypic differences or gene expression dynamics. This problem is particularly relevant for single-molecule fluorescence in situ hybridization (smFISH) studies in <i>Caenorhabditis elegans</i> embryogenesis. Microfluidics can help address this issue by allowing a large number of samples and parallelization of experiments. However, performing efficient reagent exchange on chipAnalytical chemistryHigh-Temporal-Resolution smFISH Method for Gene Expression Studies in <i>Caenorhabditis elegans</i> Embryos.PMC10619480R01 AG0564361707401R01 NS115484R35 GM119744R01 GM0883331764406R01AG056436R21NS117066R35GM119744R21 NS117066R01GM088333R01 NS096581R01NS115484R01NS0965810939511Lu HPaaby ABAubry GChou HTCharles SfalseHigh-Temporal-Resolution smFISH Method for Gene Expression Studies in <i>Caenorhabditis elegans</i> Embryos.Recent development in fluorescence-based molecular tools has contributed significantly to developmental studies, including embryogenesis. Many of these tools rely on multiple steps of sample manipulation, so obtaining large sample sizes presents a major challenge as it can be labor-intensive and time-consuming. However, large sample sizes are required to uncover critical aspects of embryogenesis, for example, subtle phenotypic differences or gene expression dynamics. This problem is particularly relevant for single-molecule fluorescence in situ hybridization (smFISH) studies in <i>Caenorhabditis elegans</i> embryogenesis. Microfluidics can help address this issue by allowing a large number of samples and parallelization of experiments. However, performing efficient reagent exchange on chip2021-01-01T00:00:00Z2021 Jan2026-06-06T21:13:09.494Z2025-04-04T14:35:49.421ZS-EPMC106194803335544910.1021/acs.analchem.0c02966