{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kazimierska M"],"funding":["Narodowe Centrum Nauki","H2020 Spreading Excellence and Widening Participation"],"pagination":["2295-2313"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10620128"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(11)"],"pubmed_abstract":["The transcription factor MYC is a proto-oncogene with a well-documented essential role in the pathogenesis and maintenance of several types of cancer. MYC binds to specific E-box sequences in the genome to regulate gene expression in a cell-type- and developmental-stage-specific manner. To date, a combined analysis of essential MYC-bound E-boxes and their downstream target genes important for growth of different types of cancer is missing. In this study, we designed a CRISPR/Cas9 library to destroy E-box sequences in a genome-wide fashion. In parallel, we used the Brunello library to knock out protein-coding genes. We performed high-throughput screens with these libraries in four MYC-dependent cancer cell lines-K562, ST486, HepG2, and MCF7-which revealed several essential E-boxes and genes"],"journal":["Molecular oncology"],"pubmed_title":["CRISPR/Cas9 screen for genome-wide interrogation of essential MYC-bound E-boxes in cancer cells."],"pmcid":["PMC10620128"],"funding_grant_id":["2016/23/D/NZ1/01611","952304","2017/26/D/NZ1/01234"],"pubmed_authors":["Sura W","Podralska M","Zurawek M","Ziolkowska-Suchanek I","van den Berg A","Kluiver J","Kazimierska M","Rozwadowska N","Wozniak T","Kasprzyk ME","Losiewski W","Dzikiewicz-Krawczyk A"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR/Cas9 screen for genome-wide interrogation of essential MYC-bound E-boxes in cancer cells.","description":"The transcription factor MYC is a proto-oncogene with a well-documented essential role in the pathogenesis and maintenance of several types of cancer. MYC binds to specific E-box sequences in the genome to regulate gene expression in a cell-type- and developmental-stage-specific manner. To date, a combined analysis of essential MYC-bound E-boxes and their downstream target genes important for growth of different types of cancer is missing. In this study, we designed a CRISPR/Cas9 library to destroy E-box sequences in a genome-wide fashion. In parallel, we used the Brunello library to knock out protein-coding genes. We performed high-throughput screens with these libraries in four MYC-dependent cancer cell lines-K562, ST486, HepG2, and MCF7-which revealed several essential E-boxes and genes","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-05-10T03:18:12.446Z","creation":"2026-05-10T03:06:11.28Z"},"accession":"S-EPMC10620128","cross_references":{"pubmed":["37519063"],"doi":["10.1002/1878-0261.13493"]}}