biostudies-literatureKazimierska MNarodowe Centrum NaukiH2020 Spreading Excellence and Widening Participation2295-2313https://www.ebi.ac.uk/biostudies/studies/S-EPMC10620128biostudies-literatureUnknown17(11)The transcription factor MYC is a proto-oncogene with a well-documented essential role in the pathogenesis and maintenance of several types of cancer. MYC binds to specific E-box sequences in the genome to regulate gene expression in a cell-type- and developmental-stage-specific manner. To date, a combined analysis of essential MYC-bound E-boxes and their downstream target genes important for growth of different types of cancer is missing. In this study, we designed a CRISPR/Cas9 library to destroy E-box sequences in a genome-wide fashion. In parallel, we used the Brunello library to knock out protein-coding genes. We performed high-throughput screens with these libraries in four MYC-dependent cancer cell lines-K562, ST486, HepG2, and MCF7-which revealed several essential E-boxes and genesMolecular oncologyCRISPR/Cas9 screen for genome-wide interrogation of essential MYC-bound E-boxes in cancer cells.PMC106201282016/23/D/NZ1/016119523042017/26/D/NZ1/01234Sura WPodralska MZurawek MZiolkowska-Suchanek Ivan den Berg AKluiver JKazimierska MRozwadowska NWozniak TKasprzyk MELosiewski WDzikiewicz-Krawczyk AfalseCRISPR/Cas9 screen for genome-wide interrogation of essential MYC-bound E-boxes in cancer cells.The transcription factor MYC is a proto-oncogene with a well-documented essential role in the pathogenesis and maintenance of several types of cancer. MYC binds to specific E-box sequences in the genome to regulate gene expression in a cell-type- and developmental-stage-specific manner. To date, a combined analysis of essential MYC-bound E-boxes and their downstream target genes important for growth of different types of cancer is missing. In this study, we designed a CRISPR/Cas9 library to destroy E-box sequences in a genome-wide fashion. In parallel, we used the Brunello library to knock out protein-coding genes. We performed high-throughput screens with these libraries in four MYC-dependent cancer cell lines-K562, ST486, HepG2, and MCF7-which revealed several essential E-boxes and genes2023-01-01T00:00:00Z2023 Nov2026-05-10T03:18:12.446Z2026-05-10T03:06:11.28ZS-EPMC106201283751906310.1002/1878-0261.13493