{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Baglamis S"],"funding":["New York Stem Cell Foundation","European Research Council","Republic of Turkey, Ministry of National Education","ZonMw","Oncode Institute"],"pagination":["18832"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10620187"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Clonal growth and competition underlie processes of key relevance in etiology, progression and therapy response across all cancers. Here, we demonstrate a novel experimental approach, based on multi-color, fluorescent tagging of cell nuclei, in combination with picoliter droplet deposition, to study the clonal dynamics in two- and three-dimensional cell cultures. The method allows for the simultaneous visualization and analysis of multiple clones in individual multi-clonal colonies, providing a powerful tool for studying clonal dynamics and identifying clonal populations with distinct characteristics. Results of our experiments validate the utility of the method in studying clonal dynamics in vitro, and reveal differences in key aspects of clonal behavior of different cancer cell lines in "],"journal":["Scientific reports"],"pubmed_title":["Using picoliter droplet deposition to track clonal competition in adherent and organoid cancer cell cultures."],"pmcid":["PMC10620187"],"funding_grant_id":["ERC-CoG 101045612","Vici 09-15018-21-10029","101045612"],"pubmed_authors":["Qian H","Lenos KJ","Vermeulen L","Baglamis S","Tans S","Krawczyk PM","Meijer D","van Zon J","Hoebe RA","Sheraton VM","Betjes MA"],"additional_accession":[]},"is_claimable":false,"name":"Using picoliter droplet deposition to track clonal competition in adherent and organoid cancer cell cultures.","description":"Clonal growth and competition underlie processes of key relevance in etiology, progression and therapy response across all cancers. Here, we demonstrate a novel experimental approach, based on multi-color, fluorescent tagging of cell nuclei, in combination with picoliter droplet deposition, to study the clonal dynamics in two- and three-dimensional cell cultures. The method allows for the simultaneous visualization and analysis of multiple clones in individual multi-clonal colonies, providing a powerful tool for studying clonal dynamics and identifying clonal populations with distinct characteristics. Results of our experiments validate the utility of the method in studying clonal dynamics in vitro, and reveal differences in key aspects of clonal behavior of different cancer cell lines in ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2025-04-05T10:23:29.99Z","creation":"2025-02-18T23:30:21.042Z"},"accession":"S-EPMC10620187","cross_references":{"pubmed":["37914743"],"doi":["10.1038/s41598-023-42849-w"]}}