{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu S"],"funding":["Hu Nan Forestry Bureau","Hunan Province Forestry Science and Technology Research and Innovation","National key R&D"],"pagination":["e0293245"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10621985"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(11)"],"pubmed_abstract":["Iron-Heart Cunninghamia lanceolata, a wild relative of Chinese fir with valuable genetic and breeding traits, has been limited in genetic studies due to a lack of genomic resources and markers. In this study, we conducted transcriptome sequencing of Iron-Heart C. lanceolata leaves using Illumina NovaSeq 6000 and performed assembly and analysis. We obtained 45,326,576 clean reads and 115,501 unigenes. Comparative analysis in five functional databases resulted in successful annotation of 26,278 unigenes, with 6,693 unigenes annotated in all databases (5.79% of the total). UniProt and Pfam databases provided annotations for 22,673 and 18,315 unigenes, respectively. Gene Ontology analysis categorized 23,962 unigenes into three categories. KEGG database alignment annotated 10,195 unigenes, clas"],"journal":["PloS one"],"pubmed_title":["De novo assembly of Iron-Heart Cunninghamia lanceolata transcriptome and EST-SSR marker development for genetic diversity analysis."],"pmcid":["PMC10621985"],"funding_grant_id":["XLKY2023-30","XLK201921","2021YFD2201303-02"],"pubmed_authors":["Zhu N","Xie G","He G","Liu S","Gong Y","Xiao C"],"additional_accession":[]},"is_claimable":false,"name":"De novo assembly of Iron-Heart Cunninghamia lanceolata transcriptome and EST-SSR marker development for genetic diversity analysis.","description":"Iron-Heart Cunninghamia lanceolata, a wild relative of Chinese fir with valuable genetic and breeding traits, has been limited in genetic studies due to a lack of genomic resources and markers. In this study, we conducted transcriptome sequencing of Iron-Heart C. lanceolata leaves using Illumina NovaSeq 6000 and performed assembly and analysis. We obtained 45,326,576 clean reads and 115,501 unigenes. Comparative analysis in five functional databases resulted in successful annotation of 26,278 unigenes, with 6,693 unigenes annotated in all databases (5.79% of the total). UniProt and Pfam databases provided annotations for 22,673 and 18,315 unigenes, respectively. Gene Ontology analysis categorized 23,962 unigenes into three categories. KEGG database alignment annotated 10,195 unigenes, clas","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-06-02T01:22:03.87Z","creation":"2025-04-05T10:23:15.962Z"},"accession":"S-EPMC10621985","cross_references":{"pubmed":["37917740"],"doi":["10.1371/journal.pone.0293245"]}}