biostudies-literatureJin JHangzhou Science and Technology Plan Development ProjectNational Key R&D Program of ChinaCAMS Innovation Fund for Medical SciencesMedical and Health Science and Technology Project of HangzhouNational Natural Science Foundation of ChinaNational Key Research and Development Program of Chinae13493https://www.ebi.ac.uk/biostudies/studies/S-EPMC10623940biostudies-literatureUnknown56(11)Cell migration and proliferation are conducive to wound healing; however, regulating cell proliferation remains challenging, and excessive proliferation is an important cause of scar hyperplasia. Here, we aimed to explore how a subvacuum environment promotes wound epithelisation without affecting scar hyperplasia. Human immortalized keratinocyte cells and human skin fibroblasts were cultured under subvacuum conditions (1/10 atmospheric pressure), and changes in cell proliferation and migration, target protein content, calcium influx, and cytoskeleton and membrane fluidity were observed. Mechanical calcium (Ca²⁺ ) channel blockers were used to prevent Ca²⁺ influx for reverse validation. A rat wound model was used to elucidate the mechanism of the subvacuum dressing in Cell proliferationSubvacuum environment-enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation.PMC106239402019YFA01106032019YFA01106022019YFA01106012019YFA0110600B20200432817721252019-I2M-5-076819300578177207620210133X01Fang HXia ZFPan BHZhu SHSun YWu GSLiu YWang KAChen YZhu LLZhu BHJin JYu SSfalseSubvacuum environment-enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation.Cell migration and proliferation are conducive to wound healing; however, regulating cell proliferation remains challenging, and excessive proliferation is an important cause of scar hyperplasia. Here, we aimed to explore how a subvacuum environment promotes wound epithelisation without affecting scar hyperplasia. Human immortalized keratinocyte cells and human skin fibroblasts were cultured under subvacuum conditions (1/10 atmospheric pressure), and changes in cell proliferation and migration, target protein content, calcium influx, and cytoskeleton and membrane fluidity were observed. Mechanical calcium (Ca²⁺ ) channel blockers were used to prevent Ca²⁺ influx for reverse validation. A rat wound model was used to elucidate the mechanism of the subvacuum dressing in 2023-01-01T00:00:00Z2023 Nov2025-04-21T23:54:07.614Z2025-04-05T19:19:41.005ZS-EPMC106239403712818010.1111/cpr.13493