biostudies-literatureXu HStrategic Priority Research Program of the Chinese Academy of SciencesNational Key Research & Developmental Program of ChinaNational Key R&D Program of ChinaNational Natural Science Foundation of China ProgramNational Key Research and Development Program of ChinaStrategic Collaborative Research Program of the Ferring Institute of Reproductive Medicinee13490https://www.ebi.ac.uk/biostudies/studies/S-EPMC10623959biostudies-literatureUnknown56(11)Despite extensive characterization of the state and function of haematopoietic stem cells (HSCs), the use of transcription factors to define the HSC population is still limited. We show here that the HSC population in mouse bone marrow can be defined by the distinct expression levels of Spi1 and Gata1. By using a double fluorescence knock-in mouse model, PGdKI, in which the expression levels of PU.1 and GATA-1 are indicated by the expression of GFP and mCherry, respectively, we uncover that the HSCs with lymphoid and myeloid repopulating activity are specifically enriched in a Lin^- PU.1^dim GATA-1^- (LPG) cell subset. In vivo competitive repopulation assays demonstrate that bone marrow cells gated by LPG exhibit haematopoietic reconstitution activity which isCell proliferationLin^- PU.1^dim GATA-1^- defines haematopoietic stem cells with long-term multilineage reconstitution activity.PMC1062395933XDA16040501317201039072018YFA0108402XDA16030302316210042022YFA110360131570995Tian JLi XCao WCao JTan SWang FZhang LWang XZhao TXu HZhao YfalseLin^- PU.1^dim GATA-1^- defines haematopoietic stem cells with long-term multilineage reconstitution activity.Despite extensive characterization of the state and function of haematopoietic stem cells (HSCs), the use of transcription factors to define the HSC population is still limited. We show here that the HSC population in mouse bone marrow can be defined by the distinct expression levels of Spi1 and Gata1. By using a double fluorescence knock-in mouse model, PGdKI, in which the expression levels of PU.1 and GATA-1 are indicated by the expression of GFP and mCherry, respectively, we uncover that the HSCs with lymphoid and myeloid repopulating activity are specifically enriched in a Lin^- PU.1^dim GATA-1^- (LPG) cell subset. In vivo competitive repopulation assays demonstrate that bone marrow cells gated by LPG exhibit haematopoietic reconstitution activity which is2023-01-01T00:00:00Z2023 Nov2025-04-21T23:50:48.372Z2025-04-05T19:22:10.151ZS-EPMC106239593714787210.1111/cpr.13490