biostudies-literatureLiu ZNIAID NIH HHSU.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious DiseasesNCI NIH HHSNIH HHS6943https://www.ebi.ac.uk/biostudies/studies/S-EPMC10628232biostudies-literatureUnknown14(1)Chromatin conformation reorganization is emerging as an important layer of regulation for gene expression and lineage specification. Yet, how lineage-specific transcription factors contribute to the establishment of cell type-specific 3D chromatin architecture in the immune cells remains unclear, especially for the late stages of T cell subset differentiation and maturation. Regulatory T cells (Treg) are mainly generated in the thymus as a subpopulation of T cells specializing in suppressing excessive immune responses. Here, by comprehensively mapping 3D chromatin organization during Treg cell differentiation, we show that Treg-specific chromatin structures were progressively established during its lineage specification, and highly associated with Treg signature gene expression. AdditionalNature communicationsFoxp3 orchestrates reorganization of chromatin architecture to establish regulatory T cell identity.PMC10628232R01 AI107027R01-AI107027R21 AI178938P30 CA014195DP5 OD023071R21 AI154919R21-AI178938U01 CA260700R01 AI151123S10 OD023689Liu ZZheng YDixon JRLiang YLee DSfalseFoxp3 orchestrates reorganization of chromatin architecture to establish regulatory T cell identity.Chromatin conformation reorganization is emerging as an important layer of regulation for gene expression and lineage specification. Yet, how lineage-specific transcription factors contribute to the establishment of cell type-specific 3D chromatin architecture in the immune cells remains unclear, especially for the late stages of T cell subset differentiation and maturation. Regulatory T cells (Treg) are mainly generated in the thymus as a subpopulation of T cells specializing in suppressing excessive immune responses. Here, by comprehensively mapping 3D chromatin organization during Treg cell differentiation, we show that Treg-specific chromatin structures were progressively established during its lineage specification, and highly associated with Treg signature gene expression. Additional2023-01-01T00:00:00Z2023 Nov2026-07-14T22:40:45.493Z2025-02-18T23:31:00.869ZS-EPMC106282323793226410.1038/s41467-023-42647-y