biostudies-literatureUnknown14Ji FAntimicrobial peptides represent a promising therapeutic alternative for the treatment of antibiotic-resistant bacterial infections. 2K4L is a rationally-designed analog of a short peptide temporin-1CEc, a natural peptide isolated and purified from the skin secretions of the Chinese brown frog <i>Rana chensinensis</i> by substituting amino acid residues. 2K4L adopt an α-helical confirm in a membrane-mimetic environment and displayed an improved and broad-spectrum antibacterial activity against sensitive and multidrug-resistant Gram-negative and Gram-positive bacterial strains. Here, the action mechanism of 2K4L on multidrug resistant <i>Acinetobacter baumannii</i> (MRAB) and protection on MRAB-infected mice was investigated. The results demonstrated high bactericidal activity of 2K4L againFrontiers in microbiology1258469https://www.ebi.ac.uk/biostudies/studies/S-EPMC10628664biostudies-literatureAntimicrobial peptide 2K4L disrupts the membrane of multidrug-resistant <i>Acinetobacter baumannii</i> and protects mice against sepsis.PMC10628664Tian GJiang FJi FShang DfalseAntimicrobial peptide 2K4L disrupts the membrane of multidrug-resistant <i>Acinetobacter baumannii</i> and protects mice against sepsis.Antimicrobial peptides represent a promising therapeutic alternative for the treatment of antibiotic-resistant bacterial infections. 2K4L is a rationally-designed analog of a short peptide temporin-1CEc, a natural peptide isolated and purified from the skin secretions of the Chinese brown frog <i>Rana chensinensis</i> by substituting amino acid residues. 2K4L adopt an α-helical confirm in a membrane-mimetic environment and displayed an improved and broad-spectrum antibacterial activity against sensitive and multidrug-resistant Gram-negative and Gram-positive bacterial strains. Here, the action mechanism of 2K4L on multidrug resistant <i>Acinetobacter baumannii</i> (MRAB) and protection on MRAB-infected mice was investigated. The results demonstrated high bactericidal activity of 2K4L again2023-01-01T00:00:00Z20232026-04-08T13:52:54.759Z2025-02-19T00:01:35.552ZS-EPMC106286643794207610.3389/fmicb.2023.1258469