{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["21(1)"],"submitter":["Zhao D"],"pubmed_abstract":["<h4>Background</h4>Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with a less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells. Here, we engineered a subset of domain-inlaid Nme2Cas9 base editors to bring the deaminase domain closer to the nontarget strand to improve editing efficiency.<h4>Results</h4>Our results demonstrated that Nme2ABE8e-797 with adenine deaminase inserted between amino acids 797 and 798 has a significantly increased editing efficiency with a wide editing window ranging from 4 to 18 bases in mammalian cells, especially at the sites that were difficult to edit by Nme2ABE8e. In addition, by swapping the PAM-interacting domain of Nme2ABE8e-797 with that"],"journal":["BMC biology"],"pagination":["250"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10636962"],"repository":["biostudies-literature"],"pubmed_title":["Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope."],"pmcid":["PMC10636962"],"pubmed_authors":["Li J","Qian Y","Zhang T","Li Z","Wang D","Hu M","Zhao D","Gao X","Lai L","Niu W","Zhou J","Xiong H"],"additional_accession":[]},"is_claimable":false,"name":"Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope.","description":"<h4>Background</h4>Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with a less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells. Here, we engineered a subset of domain-inlaid Nme2Cas9 base editors to bring the deaminase domain closer to the nontarget strand to improve editing efficiency.<h4>Results</h4>Our results demonstrated that Nme2ABE8e-797 with adenine deaminase inserted between amino acids 797 and 798 has a significantly increased editing efficiency with a wide editing window ranging from 4 to 18 bases in mammalian cells, especially at the sites that were difficult to edit by Nme2ABE8e. In addition, by swapping the PAM-interacting domain of Nme2ABE8e-797 with that","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-05-28T15:24:41.549Z","creation":"2025-02-18T23:30:34.565Z"},"accession":"S-EPMC10636962","cross_references":{"pubmed":["37946200"],"doi":["10.1186/s12915-023-01754-4"]}}