{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cardoen B"],"funding":["Natural Sciences and Engineering Research Council of Canada","Canadian Institutes of Health Research","CIHR"],"pagination":["e202206109"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10638097"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["223(1)"],"pubmed_abstract":["Identification and morphological analysis of mitochondria-ER contacts (MERCs) by fluorescent microscopy is limited by subpixel resolution interorganelle distances. Here, the membrane contact site (MCS) detection algorithm, MCS-DETECT, reconstructs subpixel resolution MERCs from 3D super-resolution image volumes. MCS-DETECT shows that elongated ribosome-studded riboMERCs, present in HT-1080 but not COS-7 cells, are morphologically distinct from smaller smooth contacts and larger contacts induced by mitochondria-ER linker expression in COS-7 cells. RiboMERC formation is associated with increased mitochondrial potential, reduced in Gp78 knockout HT-1080 cells and induced by Gp78 ubiquitin ligase activity in COS-7 and HeLa cells. Knockdown of riboMERC tether RRBP1 eliminates riboMERCs in both "],"journal":["The Journal of cell biology"],"pubmed_title":["Membrane contact site detection (MCS-DETECT) reveals dual control of rough mitochondria-ER contacts."],"pmcid":["PMC10638097"],"funding_grant_id":["RGPIN-2019-05179","AWD-022443","RGPIN-2018-03727","RGPIN-2020-06752","COP-137359","PJT-148698","COP-143083"],"pubmed_authors":["Alan P","Tiliakou E","Vandevoorde KR","Ortiz-Silva M","Vogl AW","Gao G","Cardoen B","Hamarneh G","Nabi IR","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"Membrane contact site detection (MCS-DETECT) reveals dual control of rough mitochondria-ER contacts.","description":"Identification and morphological analysis of mitochondria-ER contacts (MERCs) by fluorescent microscopy is limited by subpixel resolution interorganelle distances. Here, the membrane contact site (MCS) detection algorithm, MCS-DETECT, reconstructs subpixel resolution MERCs from 3D super-resolution image volumes. MCS-DETECT shows that elongated ribosome-studded riboMERCs, present in HT-1080 but not COS-7 cells, are morphologically distinct from smaller smooth contacts and larger contacts induced by mitochondria-ER linker expression in COS-7 cells. RiboMERC formation is associated with increased mitochondrial potential, reduced in Gp78 knockout HT-1080 cells and induced by Gp78 ubiquitin ligase activity in COS-7 and HeLa cells. Knockdown of riboMERC tether RRBP1 eliminates riboMERCs in both ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan","modification":"2026-05-28T22:30:38.755Z","creation":"2025-02-19T00:01:40.346Z"},"accession":"S-EPMC10638097","cross_references":{"pubmed":["37948126"],"doi":["10.1083/jcb.202206109"]}}