biostudies-literaturede Paiva Narciso NNIBIB NIH HHSNHLBI NIH HHSNational Institutes of HealthNIH HHSNational Science Foundatione2301265https://www.ebi.ac.uk/biostudies/studies/S-EPMC10638947biostudies-literatureUnknown12(27)Dynamic covalent chemistry (DCC) crosslinks can form hydrogels with tunable mechanical properties permissive to injectability and self-healing. However, not all hydrogels with transient crosslinks are easily extrudable. For this reason, two additional design parameters must be considered when formulating DCC-crosslinked hydrogels: 1) degree of functionalization (DoF) and 2) polymer molecular weight (MW). To investigate these parameters, hydrogels comprised of two recombinant biopolymers: 1) a hyaluronic acid (HA) modified with benzaldehyde and 2) an elastin-like protein (ELP) modified with hydrazine (ELP-HYD), are formulated. Several hydrogel families are synthesized with distinct HA MW and DoF while keeping the ELP-HYD component constant. The resulting hydrogels have a range of stiffnesseAdvanced healthcare materialsDesign Parameters for Injectable Biopolymeric Hydrogels with Dynamic Covalent Chemistry Crosslinks.PMC10638947R01 EB027171R01 HL142718R25 HL147666R01 HL151997CBET2033302R01HL151997R01 EB027666Gilchrist AETrigo MLMNavarro RSAviles Rodriguez GHeilshorn SCde Paiva Narciso NfalseDesign Parameters for Injectable Biopolymeric Hydrogels with Dynamic Covalent Chemistry Crosslinks.Dynamic covalent chemistry (DCC) crosslinks can form hydrogels with tunable mechanical properties permissive to injectability and self-healing. However, not all hydrogels with transient crosslinks are easily extrudable. For this reason, two additional design parameters must be considered when formulating DCC-crosslinked hydrogels: 1) degree of functionalization (DoF) and 2) polymer molecular weight (MW). To investigate these parameters, hydrogels comprised of two recombinant biopolymers: 1) a hyaluronic acid (HA) modified with benzaldehyde and 2) an elastin-like protein (ELP) modified with hydrazine (ELP-HYD), are formulated. Several hydrogel families are synthesized with distinct HA MW and DoF while keeping the ELP-HYD component constant. The resulting hydrogels have a range of stiffnesse2023-01-01T00:00:00Z2023 Oct2025-04-04T01:59:32.413Z2025-04-04T01:59:32.413ZS-EPMC106389473738981110.1002/adhm.202301265