{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sun Y"],"funding":["Deutsche Forschungsgemeinschaft","Chinese Scholarship Council","Klaus Tschira Foundation"],"pagination":["11197-11212"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10639084"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["51(20)"],"pubmed_abstract":["Queuosine (Q) is a complex tRNA modification found in bacteria and eukaryotes at position 34 of four tRNAs with a GUN anticodon, and it regulates the translational efficiency and fidelity of the respective codons that differ at the Wobble position. In bacteria, the biosynthesis of Q involves two precursors, preQ0 and preQ1, whereas eukaryotes directly obtain Q from bacterial sources. The study of queuosine has been challenging due to the limited availability of high-throughput methods for its detection and analysis. Here, we have employed direct RNA sequencing using nanopore technology to detect the modification of tRNAs with Q and Q precursors. These modifications were detected with high accuracy on synthetic tRNAs as well as on tRNAs extracted from Schizosaccharomyces pombe and Escherich"],"journal":["Nucleic acids research"],"pubmed_title":["Detection of queuosine and queuosine precursors in tRNAs by direct RNA sequencing."],"pmcid":["PMC10639084"],"funding_grant_id":["EH237/ 13-2","SPP1784","00.013.2021","EH237/ 19-1"],"pubmed_authors":["Naarmann-de Vries I","Piechotta M","Dieterich C","Sun Y","Ehrenhofer-Murray AE"],"additional_accession":[]},"is_claimable":false,"name":"Detection of queuosine and queuosine precursors in tRNAs by direct RNA sequencing.","description":"Queuosine (Q) is a complex tRNA modification found in bacteria and eukaryotes at position 34 of four tRNAs with a GUN anticodon, and it regulates the translational efficiency and fidelity of the respective codons that differ at the Wobble position. In bacteria, the biosynthesis of Q involves two precursors, preQ0 and preQ1, whereas eukaryotes directly obtain Q from bacterial sources. The study of queuosine has been challenging due to the limited availability of high-throughput methods for its detection and analysis. Here, we have employed direct RNA sequencing using nanopore technology to detect the modification of tRNAs with Q and Q precursors. These modifications were detected with high accuracy on synthetic tRNAs as well as on tRNAs extracted from Schizosaccharomyces pombe and Escherich","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-06-23T03:20:39.082Z","creation":"2025-04-04T13:15:41.742Z"},"accession":"S-EPMC10639084","cross_references":{"pubmed":["37811872"],"doi":["10.1093/nar/gkad826"]}}