{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao H"],"funding":["Science and Technology Innovation Plan Of Shanghai Science and Technology Commission","Natural Science Foundation of Shandong Province","National Natural Science Foundation of China"],"pagination":["2739-2750"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10643753"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["40(11)"],"pubmed_abstract":["Purpose
To investigate the expression and underlying mechanism of RPA2 in endometrium of patients with repeated implantation failure (RIF).Methods
In this study, we retrieved the expression profiles from GEO databases and filtered the differentially expressed genes between RIF and the fertile control group. Ultimately, RPA2 was confirmed as a target gene. RPA2 expression in endometrial tissues of RIF patients, the control group, and different phases was detected by RT-qPCR, immunohistochemistry, and Western blotting. The role of RPA2 in endometrial decidualization was performed by in vitro decidualization inducing by 8-Br-cAMP and MPA. Furthermore, RT-qPCR was used to detect changes in the decidual biomarkers after transfection of RPA2 overexpression vector in human endomet"],"journal":["Journal of assisted reproduction and genetics"],"pubmed_title":["Upregulated RPA2 in endometrial tissues of repeated implantation failure patients impairs the endometrial decidualization."],"pmcid":["PMC10643753"],"funding_grant_id":["2020MSGY089 and 2022YD031","ZR2021MH012","82101698"],"pubmed_authors":["Liu X","Bao H","Chen G","Cong J","Lv N","Zhao H"],"additional_accession":[]},"is_claimable":false,"name":"Upregulated RPA2 in endometrial tissues of repeated implantation failure patients impairs the endometrial decidualization.","description":"Purpose
To investigate the expression and underlying mechanism of RPA2 in endometrium of patients with repeated implantation failure (RIF).Methods
In this study, we retrieved the expression profiles from GEO databases and filtered the differentially expressed genes between RIF and the fertile control group. Ultimately, RPA2 was confirmed as a target gene. RPA2 expression in endometrial tissues of RIF patients, the control group, and different phases was detected by RT-qPCR, immunohistochemistry, and Western blotting. The role of RPA2 in endometrial decidualization was performed by in vitro decidualization inducing by 8-Br-cAMP and MPA. Furthermore, RT-qPCR was used to detect changes in the decidual biomarkers after transfection of RPA2 overexpression vector in human endomet","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-05-28T03:19:30.979Z","creation":"2025-04-04T22:47:44.302Z"},"accession":"S-EPMC10643753","cross_references":{"pubmed":["37831348"],"doi":["10.1007/s10815-023-02946-1"]}}