biostudies-literatureZhao HScience and Technology Innovation Plan Of Shanghai Science and Technology CommissionNatural Science Foundation of Shandong ProvinceNational Natural Science Foundation of China2739-2750https://www.ebi.ac.uk/biostudies/studies/S-EPMC10643753biostudies-literatureUnknown40(11)<h4>Purpose</h4>To investigate the expression and underlying mechanism of RPA2 in endometrium of patients with repeated implantation failure (RIF).<h4>Methods</h4>In this study, we retrieved the expression profiles from GEO databases and filtered the differentially expressed genes between RIF and the fertile control group. Ultimately, RPA2 was confirmed as a target gene. RPA2 expression in endometrial tissues of RIF patients, the control group, and different phases was detected by RT-qPCR, immunohistochemistry, and Western blotting. The role of RPA2 in endometrial decidualization was performed by in vitro decidualization inducing by 8-Br-cAMP and MPA. Furthermore, RT-qPCR was used to detect changes in the decidual biomarkers after transfection of RPA2 overexpression vector in human endometJournal of assisted reproduction and geneticsUpregulated RPA2 in endometrial tissues of repeated implantation failure patients impairs the endometrial decidualization.PMC106437532020MSGY089 and 2022YD031ZR2021MH01282101698Liu XBao HChen GCong JLv NZhao HfalseUpregulated RPA2 in endometrial tissues of repeated implantation failure patients impairs the endometrial decidualization.<h4>Purpose</h4>To investigate the expression and underlying mechanism of RPA2 in endometrium of patients with repeated implantation failure (RIF).<h4>Methods</h4>In this study, we retrieved the expression profiles from GEO databases and filtered the differentially expressed genes between RIF and the fertile control group. Ultimately, RPA2 was confirmed as a target gene. RPA2 expression in endometrial tissues of RIF patients, the control group, and different phases was detected by RT-qPCR, immunohistochemistry, and Western blotting. The role of RPA2 in endometrial decidualization was performed by in vitro decidualization inducing by 8-Br-cAMP and MPA. Furthermore, RT-qPCR was used to detect changes in the decidual biomarkers after transfection of RPA2 overexpression vector in human endomet2023-01-01T00:00:00Z2023 Nov2026-05-28T03:19:30.979Z2025-04-04T22:47:44.302ZS-EPMC106437533783134810.1007/s10815-023-02946-1